tetano
Editor, Senior Moderator
Jundishapur J Microbiol. 2015 Aug 1;8(8):e19041. doi: 10.5812/jjm.19041v2. eCollection 2015.
[h=1]Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses.[/h] Sultani M[SUP]1[/SUP], Mokhtari Azad T[SUP]1[/SUP], Eshragian M[SUP]1[/SUP], Shadab A[SUP]1[/SUP], Naseri M[SUP]1[/SUP], Eilami O[SUP]2[/SUP], Yavarian J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.
[h=4]OBJECTIVES:[/h] The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR Green real-time polymerase chain reaction (PCR).
[h=4]MATERIALS AND METHODS:[/h] Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR Green multiplex real-time PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43.
[h=4]RESULTS:[/h] Application of the multiplex SYBR Green real-time PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012).
[h=4]CONCLUSIONS:[/h] Multiplex SYBR Green real-time PCR is a rapid and relatively inexpensive method for detection of respiratory viruses.
[h=4]KEYWORDS:[/h] Real-Time PCR; Respiratory Syncytial Viruses; SYBR Green
PMID: 26468358 [PubMed]
[h=1]Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses.[/h] Sultani M[SUP]1[/SUP], Mokhtari Azad T[SUP]1[/SUP], Eshragian M[SUP]1[/SUP], Shadab A[SUP]1[/SUP], Naseri M[SUP]1[/SUP], Eilami O[SUP]2[/SUP], Yavarian J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.
[h=4]OBJECTIVES:[/h] The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR Green real-time polymerase chain reaction (PCR).
[h=4]MATERIALS AND METHODS:[/h] Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR Green multiplex real-time PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43.
[h=4]RESULTS:[/h] Application of the multiplex SYBR Green real-time PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012).
[h=4]CONCLUSIONS:[/h] Multiplex SYBR Green real-time PCR is a rapid and relatively inexpensive method for detection of respiratory viruses.
[h=4]KEYWORDS:[/h] Real-Time PCR; Respiratory Syncytial Viruses; SYBR Green
PMID: 26468358 [PubMed]