tetano
Editor, Senior Moderator
Arch Virol. 2015 Dec 23. [Epub ahead of print]
[h=1]Multiple amino acid substitutions involved in the adaptation of avian-origin influenza A (H10N7) virus in mice.[/h] Wu H[SUP]1[/SUP], Peng X[SUP]1[/SUP], Peng X[SUP]1[/SUP], Cheng L[SUP]1[/SUP], Jin C[SUP]1[/SUP], Lu X[SUP]1[/SUP], Xie T[SUP]1[/SUP], Yao H[SUP]1[/SUP], Wu N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To identify substitutions that are possibly associated with the adaptation of avian-origin H10N7 virus to mammals, adaptation of the H10N7 virus in mouse lung was carried out by serial lung-to-lung passage. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E627K), PA (T97I), and HA (G409E) proteins, and this virus was more virulent in mice than the wild-type virus. Our results suggest that these substitutions are involved in the enhancement of the replication efficiency of avian-origin H10N7 virus, resulting in severe disease in mice. Continued poultry surveillance of these substitutions in H10N7 viruses is required.
[h=4]KEYWORDS:[/h] Adaptation; Avian influenza viruses; Mice; Replication; Subtype H10N7; Virulence
PMID: 26699787 [PubMed - as supplied by publisher]
[h=1]Multiple amino acid substitutions involved in the adaptation of avian-origin influenza A (H10N7) virus in mice.[/h] Wu H[SUP]1[/SUP], Peng X[SUP]1[/SUP], Peng X[SUP]1[/SUP], Cheng L[SUP]1[/SUP], Jin C[SUP]1[/SUP], Lu X[SUP]1[/SUP], Xie T[SUP]1[/SUP], Yao H[SUP]1[/SUP], Wu N[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To identify substitutions that are possibly associated with the adaptation of avian-origin H10N7 virus to mammals, adaptation of the H10N7 virus in mouse lung was carried out by serial lung-to-lung passage. Genomic analysis of the mouse-adapted virus revealed amino acid changes in the PB2 (E627K), PA (T97I), and HA (G409E) proteins, and this virus was more virulent in mice than the wild-type virus. Our results suggest that these substitutions are involved in the enhancement of the replication efficiency of avian-origin H10N7 virus, resulting in severe disease in mice. Continued poultry surveillance of these substitutions in H10N7 viruses is required.
[h=4]KEYWORDS:[/h] Adaptation; Avian influenza viruses; Mice; Replication; Subtype H10N7; Virulence
PMID: 26699787 [PubMed - as supplied by publisher]