tetano
Editor, Senior Moderator
Viruses. 2015 Oct 13;7(10):5319-27. doi: 10.3390/v7102873.
[h=1]Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses.[/h] Tran V[SUP]1,[/SUP][SUP]2[/SUP], Poole DS[SUP]3[/SUP], Jeffery JJ[SUP]4[/SUP], Sheahan TP[SUP]5[/SUP], Creech D[SUP]6[/SUP], Yevtodiyenko A[SUP]7[/SUP], Peat AJ[SUP]8[/SUP], Francis KP[SUP]9[/SUP], You S[SUP]10[/SUP], Mehle A[SUP]11[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
[h=4]KEYWORDS:[/h] influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus
PMID: 26473913 [PubMed - in process] Free full text
[h=1]Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses.[/h] Tran V[SUP]1,[/SUP][SUP]2[/SUP], Poole DS[SUP]3[/SUP], Jeffery JJ[SUP]4[/SUP], Sheahan TP[SUP]5[/SUP], Creech D[SUP]6[/SUP], Yevtodiyenko A[SUP]7[/SUP], Peat AJ[SUP]8[/SUP], Francis KP[SUP]9[/SUP], You S[SUP]10[/SUP], Mehle A[SUP]11[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
[h=4]KEYWORDS:[/h] influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus
PMID: 26473913 [PubMed - in process] Free full text