tetano
Editor, Senior Moderator
J Ethnopharmacol. 2015 Dec 21. pii: S0378-8741(15)30284-1. doi: 10.1016/j.jep.2015.12.034. [Epub ahead of print]
[h=1]Mosla scabra flavonoids ameliorate the influenza A virus-induced lung injury and water transport abnormality via the inhibition of PRR and AQP signaling pathways in mice.[/h] Yu CH[SUP]1[/SUP], Yu WY[SUP]1[/SUP], Fang J[SUP]1[/SUP], Zhang HH[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Yu B[SUP]2[/SUP], Wu F[SUP]3[/SUP], Wu XN[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]ETHNOPHARMACROLOGICAL RELEVANCE:[/h] Mosla scabra (Thunb.) C.Y. Wu & H. W. Li has been used as a traditional medicinal herb for centuries in East Asian countries. It has antibacterial, antiviral, antioxidant, anti-inflammatory and immunomodulatory effects. In folk medicine, it is used as a remedy for the treatment of pulmonary diseases, such as fever, cold, cough, pulmonary edema and emphysema.
[h=4]AIM OF THE STUDY:[/h] This study was to investigate the protective mechanisms of total flavonoids from M. scabra (MF) in influenza A virus (IAV)-infected mice.
[h=4]MATERIALS AND METHODS:[/h] The mice were infected with IAV and then were treated daily with MF for five days. At the end of the experiment, the levels of inflammatory-related cytokines (IFN-α, IL-6, TNF-α and IL-1β) were determined by ELISA. Pathological changes of lung tissue were examined by H&E staining. The protein expressions of AQP5, p-p38, caspase-3 and NF-κB p65 were detected by western blot analysis while the gene expressions of key effectors in AQP5 and PRRs signaling pathways were detected by real-time Fluorescence Quantitative Polymerase Chain Reaction (RFQ-PCR) analysis.
[h=4]RESULTS:[/h] The results showed that treatment with MF at doses of 120-360mg/kg for five days to IAV-infected mice significantly attenuated IAV-induced pulmonary injury and decreased the serum levels of IL-6, TNF-α and IL-1β, but increased IFN-α levels. MF treatment could up-regulate the mRNA expressions of TLR-7, RIG-1, TRAF6, Bcl-2, Bax, VIPR1, PKCα and AQP5 and down-regulate caspase-3 and NF-κB p65 protein expression.
[h=4]CONCLUSION:[/h] Treatment with MF could significantly alleviate IAV-induced pulmonary inflammation, apoptosis and water transport abnormality, which was probably through the regulation of TLR7, RIG-1 and AQP5 signaling pathway.
Copyright ? 2015. Published by Elsevier Ireland Ltd.
[h=4]KEYWORDS:[/h] Antiviral; Apoptosis; Aquaporin 5; Inflammation; Vasoactive intestinal peptide
PMID: 26719287 [PubMed - as supplied by publisher]
[h=1]Mosla scabra flavonoids ameliorate the influenza A virus-induced lung injury and water transport abnormality via the inhibition of PRR and AQP signaling pathways in mice.[/h] Yu CH[SUP]1[/SUP], Yu WY[SUP]1[/SUP], Fang J[SUP]1[/SUP], Zhang HH[SUP]1[/SUP], Ma Y[SUP]1[/SUP], Yu B[SUP]2[/SUP], Wu F[SUP]3[/SUP], Wu XN[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]ETHNOPHARMACROLOGICAL RELEVANCE:[/h] Mosla scabra (Thunb.) C.Y. Wu & H. W. Li has been used as a traditional medicinal herb for centuries in East Asian countries. It has antibacterial, antiviral, antioxidant, anti-inflammatory and immunomodulatory effects. In folk medicine, it is used as a remedy for the treatment of pulmonary diseases, such as fever, cold, cough, pulmonary edema and emphysema.
[h=4]AIM OF THE STUDY:[/h] This study was to investigate the protective mechanisms of total flavonoids from M. scabra (MF) in influenza A virus (IAV)-infected mice.
[h=4]MATERIALS AND METHODS:[/h] The mice were infected with IAV and then were treated daily with MF for five days. At the end of the experiment, the levels of inflammatory-related cytokines (IFN-α, IL-6, TNF-α and IL-1β) were determined by ELISA. Pathological changes of lung tissue were examined by H&E staining. The protein expressions of AQP5, p-p38, caspase-3 and NF-κB p65 were detected by western blot analysis while the gene expressions of key effectors in AQP5 and PRRs signaling pathways were detected by real-time Fluorescence Quantitative Polymerase Chain Reaction (RFQ-PCR) analysis.
[h=4]RESULTS:[/h] The results showed that treatment with MF at doses of 120-360mg/kg for five days to IAV-infected mice significantly attenuated IAV-induced pulmonary injury and decreased the serum levels of IL-6, TNF-α and IL-1β, but increased IFN-α levels. MF treatment could up-regulate the mRNA expressions of TLR-7, RIG-1, TRAF6, Bcl-2, Bax, VIPR1, PKCα and AQP5 and down-regulate caspase-3 and NF-κB p65 protein expression.
[h=4]CONCLUSION:[/h] Treatment with MF could significantly alleviate IAV-induced pulmonary inflammation, apoptosis and water transport abnormality, which was probably through the regulation of TLR7, RIG-1 and AQP5 signaling pathway.
Copyright ? 2015. Published by Elsevier Ireland Ltd.
[h=4]KEYWORDS:[/h] Antiviral; Apoptosis; Aquaporin 5; Inflammation; Vasoactive intestinal peptide
PMID: 26719287 [PubMed - as supplied by publisher]