tetano
Editor, Senior Moderator
Exp Hematol. 2015 Mar 17. pii: S0301-472X(15)00092-2. doi: 10.1016/j.exphem.2015.02.005. [Epub ahead of print]
[h=1]Monoclonal paraprotein influences baseline B cell repertoire diversity and pertubates influenza vaccination-induced B cell response.[/h] Tete SM[SUP]1[/SUP], Kipling D[SUP]2[/SUP], Westra J[SUP]3[/SUP], de Haan A[SUP]4[/SUP], Bijl M[SUP]5[/SUP], Dunn-Walters DK[SUP]6[/SUP], Sahota SS[SUP]7[/SUP], Bos NA[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Monoclonal gammopathy of undetermined significance (MGUS) arises from a clonal expansion of plasma cells in the bone marrow, secreting monoclonal (M) paraprotein. MGUS is associated with increased susceptibility to infections, which may reflect altered B-cell repertoire. To investigate this, we examined the IgM, IgG and IgA B-cell repertoire diversity in MGUS at baseline and after influenza vaccination (n=16) in comparison to healthy controls (HCs) (n=16). The CDR3 region of the immunoglobulin heavy chain variable (IGHV) region gene was amplified and B-cell spectratypes analyzed by high-resolution electrophoresis. Spectratype Gaussian distribution, kurtosis and skewness were quantified to measure repertoire shifts. Both HC and MGUS baseline spectratypes show inter-individual variability that is more pronounced in the IGHG and IGHA repertoires. Overall, baseline B cell repertoire is more altered in MGUS, with oligoclonality observed in 50% (p=0.01). Post-vaccination, significant differences emerged in MGUS in relation to M-protein levels. High M-protein concentration is associated with a more oligoclonal IgG and IgA response at day 7 post-vaccination, and in contrast to HCs, vaccination also induced significant perturbations in the MGUS IgM repertoire at day 7 (p=0.005). Monoclonal expansion in MGUS thus has an effect on the baseline B cell repertoire and influences the recruited repertoire upon vaccination.
Copyright ? 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] B cell repertoire; influenza vaccination; monoclonal gammopathy of undetermined significance; monoclonal protein
PMID: 25795522 [PubMed - as supplied by publisher]
[h=1]Monoclonal paraprotein influences baseline B cell repertoire diversity and pertubates influenza vaccination-induced B cell response.[/h] Tete SM[SUP]1[/SUP], Kipling D[SUP]2[/SUP], Westra J[SUP]3[/SUP], de Haan A[SUP]4[/SUP], Bijl M[SUP]5[/SUP], Dunn-Walters DK[SUP]6[/SUP], Sahota SS[SUP]7[/SUP], Bos NA[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Monoclonal gammopathy of undetermined significance (MGUS) arises from a clonal expansion of plasma cells in the bone marrow, secreting monoclonal (M) paraprotein. MGUS is associated with increased susceptibility to infections, which may reflect altered B-cell repertoire. To investigate this, we examined the IgM, IgG and IgA B-cell repertoire diversity in MGUS at baseline and after influenza vaccination (n=16) in comparison to healthy controls (HCs) (n=16). The CDR3 region of the immunoglobulin heavy chain variable (IGHV) region gene was amplified and B-cell spectratypes analyzed by high-resolution electrophoresis. Spectratype Gaussian distribution, kurtosis and skewness were quantified to measure repertoire shifts. Both HC and MGUS baseline spectratypes show inter-individual variability that is more pronounced in the IGHG and IGHA repertoires. Overall, baseline B cell repertoire is more altered in MGUS, with oligoclonality observed in 50% (p=0.01). Post-vaccination, significant differences emerged in MGUS in relation to M-protein levels. High M-protein concentration is associated with a more oligoclonal IgG and IgA response at day 7 post-vaccination, and in contrast to HCs, vaccination also induced significant perturbations in the MGUS IgM repertoire at day 7 (p=0.005). Monoclonal expansion in MGUS thus has an effect on the baseline B cell repertoire and influences the recruited repertoire upon vaccination.
Copyright ? 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] B cell repertoire; influenza vaccination; monoclonal gammopathy of undetermined significance; monoclonal protein
PMID: 25795522 [PubMed - as supplied by publisher]