Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 20

[tetano]

J Virol Methods. 2012 May 29. [Epub ahead of print]
Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 2010-2011.
Arvia R, Corcioli F, Simi L, Orlando C, De Santis R, Facchini M, Donatelli I, Azzi A.
Source

Public Health Department, University of Florence, Italy.
Abstract

For the early detection of the H275Y mutation as a marker of oseltamivir resistance in A(H1N1) pandemic strains, a sensitive and specific pyrosequencing assay was developed. This assay analyses a region 99 nts long, encompassing the H275Y site, amplified by a nested PCR. Seventy five respiratory specimens, obtained from 62 patients during the pandemic and in the 2010-2011 influenza season, in Tuscany, were tested. Resistant strains were demonstrated in 10 patients. In three other patients, resistant and sensitive variants were found. This pyrosequencing assay may be a useful method for monitoring the spread of resistant influenza H1N1 2009 strains.

Copyright ? 2012. Published by Elsevier B.V.

PMID:
22659066
[PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/22659066