tetano
Editor, Senior Moderator
J Med Virol. 2019 Aug 7. doi: 10.1002/jmv.25565. [Epub ahead of print]
[h=1]Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015-2016 season and comparison of HA mutations detected in Turkey since 2009.[/h] Guldemir D[SUP]1[/SUP], Coskun-Ari FF[SUP]1[/SUP], Altas AB[SUP]2[/SUP], Bakkaloglu Z[SUP]1[/SUP], Unaldi O[SUP]1[/SUP], Bayraktar F[SUP]2[/SUP], Korukluoglu G[SUP]2[/SUP], Aktas AR[SUP]1[/SUP], Durmaz R[SUP]3[/SUP].
[h=3]Author information[/h] 1 National Molecular Microbiology Reference Laboratory, Public Health General Directorate, Ministry of Health, Ankara, Turkey. 2 National Viral Respiratory Pathogens Reference Laboratory, Public Health General Directorate, Ministry of Health, Ankara, Turkey. 3 Department of Clinical Microbiology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
[h=3]Abstract[/h] Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post-pandemic period, but its variants continued to be the prevailing subtype in the 2015-2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015-2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase (NA) genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99,5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96,6%-97,2%) was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Over all 14 common mutations were detected in HA sequences of all 2015-2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in twelve HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir-sensitive genotype, suggesting that Tamiflu[SUP]?[/SUP] (Oseltamivir) could still be the drug of choice in Turkey. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] 2015-2016 influenza season; HA; Influenza A(H1N1)pdm09; NA; Oseltamivir resistance
PMID: 31389035 DOI: 10.1002/jmv.25565
[h=1]Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015-2016 season and comparison of HA mutations detected in Turkey since 2009.[/h] Guldemir D[SUP]1[/SUP], Coskun-Ari FF[SUP]1[/SUP], Altas AB[SUP]2[/SUP], Bakkaloglu Z[SUP]1[/SUP], Unaldi O[SUP]1[/SUP], Bayraktar F[SUP]2[/SUP], Korukluoglu G[SUP]2[/SUP], Aktas AR[SUP]1[/SUP], Durmaz R[SUP]3[/SUP].
[h=3]Author information[/h] 1 National Molecular Microbiology Reference Laboratory, Public Health General Directorate, Ministry of Health, Ankara, Turkey. 2 National Viral Respiratory Pathogens Reference Laboratory, Public Health General Directorate, Ministry of Health, Ankara, Turkey. 3 Department of Clinical Microbiology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
[h=3]Abstract[/h] Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post-pandemic period, but its variants continued to be the prevailing subtype in the 2015-2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015-2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase (NA) genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99,5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96,6%-97,2%) was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Over all 14 common mutations were detected in HA sequences of all 2015-2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in twelve HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir-sensitive genotype, suggesting that Tamiflu[SUP]?[/SUP] (Oseltamivir) could still be the drug of choice in Turkey. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] 2015-2016 influenza season; HA; Influenza A(H1N1)pdm09; NA; Oseltamivir resistance
PMID: 31389035 DOI: 10.1002/jmv.25565