tetano
Editor, Senior Moderator
Emerg Microbes Infect. 2016 Jul 20;5(7):e73. doi: 10.1038/emi.2016.71.
[h=1]Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011.[/h] Zou S[SUP]1[/SUP], Gao R[SUP]1[/SUP], Zhang Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Chen W[SUP]1[/SUP], Bai T[SUP]1[/SUP], Dong L[SUP]1[/SUP], Wang D[SUP]1[/SUP], Shu Y[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H6 avian influenza viruses (AIVs), which are prevalent in domestic and wild birds in Eurasian countries, have been isolated from pigs, a dog and a human. Routine virological surveillance at live poultry markets or poultry farms was conducted in southern China from 2009 to 2011. This study investigated the genetic and antigenic characteristics, analyzed the receptor-binding properties and evaluated the kinetics of infectivity of the AIVs in A549, MDCK and PK15 cells. A total of 14 H6N6 and 2 H6N2 isolates were obtained from four provinces in southern China. Genetic analysis indicated two distinct hemagglutinin lineages of the H6 strains cocirculating in southern China, and these strains facilitated active evolution and reassortment among multiple influenza virus subtypes from different avian species in nature. None of these isolates grouped with the novel Taiwan H6N1 virus responsible for human infection. Receptor-binding specificity assays showed that five H6 AIVs may have acquired the ability to recognize human receptors. Growth kinetics experiments showed that EV/HB-JZ/02/10(H6N2) and EV/JX/15/10(H6N6) initially reproduced faster and achieved higher titers than other viruses, suggesting that enhanced binding to α-2,6-linked sialic acids correlated with increased viral replication in mammalian cells. Overall, the results emphasize the need for continued surveillance of H6 outbreaks and extensive characterization of H6 isolates to better understand genetic changes and their implications.
PMID: 27436363 DOI: 10.1038/emi.2016.71
[PubMed - in process]
[h=1]Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011.[/h] Zou S[SUP]1[/SUP], Gao R[SUP]1[/SUP], Zhang Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Chen W[SUP]1[/SUP], Bai T[SUP]1[/SUP], Dong L[SUP]1[/SUP], Wang D[SUP]1[/SUP], Shu Y[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] H6 avian influenza viruses (AIVs), which are prevalent in domestic and wild birds in Eurasian countries, have been isolated from pigs, a dog and a human. Routine virological surveillance at live poultry markets or poultry farms was conducted in southern China from 2009 to 2011. This study investigated the genetic and antigenic characteristics, analyzed the receptor-binding properties and evaluated the kinetics of infectivity of the AIVs in A549, MDCK and PK15 cells. A total of 14 H6N6 and 2 H6N2 isolates were obtained from four provinces in southern China. Genetic analysis indicated two distinct hemagglutinin lineages of the H6 strains cocirculating in southern China, and these strains facilitated active evolution and reassortment among multiple influenza virus subtypes from different avian species in nature. None of these isolates grouped with the novel Taiwan H6N1 virus responsible for human infection. Receptor-binding specificity assays showed that five H6 AIVs may have acquired the ability to recognize human receptors. Growth kinetics experiments showed that EV/HB-JZ/02/10(H6N2) and EV/JX/15/10(H6N6) initially reproduced faster and achieved higher titers than other viruses, suggesting that enhanced binding to α-2,6-linked sialic acids correlated with increased viral replication in mammalian cells. Overall, the results emphasize the need for continued surveillance of H6 outbreaks and extensive characterization of H6 isolates to better understand genetic changes and their implications.
PMID: 27436363 DOI: 10.1038/emi.2016.71
[PubMed - in process]