tetano
Editor, Senior Moderator
Mol Biol Cell
. 2021 May 19;mbcE21020074.
doi: 10.1091/mbc.E21-02-0074. Online ahead of print.
Peroxisomes exhibit compromised structure and matrix protein content in SARS-CoV-2-infected cells
Barbara Knoblach[SUP] 1 [/SUP], Ray Ishida[SUP] 2 [/SUP], Tom C Hobman[SUP] 1 2 3 [/SUP], Richard A Rachubinski[SUP] 1 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has triggered global health and economic crises. Here we report the effects of SARS-CoV-2 infection on peroxisomes of human cell lines, Huh-7 and SK-N-SH. Peroxisomes undergo dramatic changes in morphology in SARS-CoV-2-infected cells. Rearrangement of peroxisomal membranes is followed by redistribution of peroxisomal matrix proteins to the cytosol, resulting in a dramatic decrease in the numbers of mature peroxisomes. The SARS-CoV-2 ORF14 protein was shown to interact physically with human PEX14, a peroxisomal membrane protein required for matrix protein import and peroxisome biogenesis. Given the important roles of peroxisomes in innate immunity, SARS-CoV-2 may directly target peroxisomes, resulting in loss of peroxisome structural integrity, matrix protein content and ability to function in antiviral signaling. [Media: see text].
. 2021 May 19;mbcE21020074.
doi: 10.1091/mbc.E21-02-0074. Online ahead of print.
Peroxisomes exhibit compromised structure and matrix protein content in SARS-CoV-2-infected cells
Barbara Knoblach[SUP] 1 [/SUP], Ray Ishida[SUP] 2 [/SUP], Tom C Hobman[SUP] 1 2 3 [/SUP], Richard A Rachubinski[SUP] 1 [/SUP]
Affiliations
- PMID: 34010015
- DOI: 10.1091/mbc.E21-02-0074
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has triggered global health and economic crises. Here we report the effects of SARS-CoV-2 infection on peroxisomes of human cell lines, Huh-7 and SK-N-SH. Peroxisomes undergo dramatic changes in morphology in SARS-CoV-2-infected cells. Rearrangement of peroxisomal membranes is followed by redistribution of peroxisomal matrix proteins to the cytosol, resulting in a dramatic decrease in the numbers of mature peroxisomes. The SARS-CoV-2 ORF14 protein was shown to interact physically with human PEX14, a peroxisomal membrane protein required for matrix protein import and peroxisome biogenesis. Given the important roles of peroxisomes in innate immunity, SARS-CoV-2 may directly target peroxisomes, resulting in loss of peroxisome structural integrity, matrix protein content and ability to function in antiviral signaling. [Media: see text].