tetano
Editor, Senior Moderator
NPJ Vaccines. 2018 Jul 3;3:25. doi: 10.1038/s41541-018-0063-7. eCollection 2018.
[h=1]Mini-hemagglutinin vaccination induces cross-reactive antibodies in pre-exposed NHP that protect mice against lethal influenza challenge.[/h] van der Lubbe JEM[SUP]1[/SUP], Huizingh J[SUP]1[/SUP], Verspuij JWA[SUP]1[/SUP], Tettero L[SUP]1[/SUP], Schmit-Tillemans SPR[SUP]1[/SUP], Mooij P[SUP]2[/SUP], Mortier D[SUP]2[/SUP], Koopman G[SUP]2[/SUP], Bogers WMJM[SUP]2[/SUP], Dekking L[SUP]1[/SUP], Meijberg W[SUP]1,[/SUP][SUP]3[/SUP], Kwaks T[SUP]1[/SUP], Brandenburg B[SUP]1[/SUP], Tolboom JTBM[SUP]1[/SUP], Schuitemaker H[SUP]1[/SUP], Roozendaal R[SUP]1[/SUP], Kuipers H[SUP]#[/SUP][SUP]1[/SUP], Zahn RC[SUP]#[/SUP][SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Seasonal vaccines are currently the most effective countermeasure against influenza. However, seasonal vaccines are only effective against strains closely related to the influenza strains contained in the vaccine. Recently a new hemagglutinin (HA) stem-based antigen, the so-called "mini-HA", has been shown to induce a cross-protective immune response in influenza-naive mice and non-human primates (NHP). However, prior exposure to influenza can have a profound effect on the immune response to subsequent influenza infection and the protective efficacy of vaccination. Here we show that mini-HA, compared to a trivalent influenza vaccine (TIV), elicits a broadened influenza-specific humoral immune response in NHP previously exposed to influenza. Serum transfer experiments showed that antibodies induced by both mini-HA and seasonal vaccine protected mice against lethal challenge with a H1N1 influenza strain heterologous to the H1 HA included in the TIV. However, antibodies elicited by mini-HA showed an additional benefit of protecting mice against lethal heterosubtypic H5N1 influenza challenge, associated with H5 HA-specific functional antibodies.
PMID: 29977611 PMCID: PMC6030213 DOI: 10.1038/s41541-018-0063-7
Free PMC Article
[h=1]Mini-hemagglutinin vaccination induces cross-reactive antibodies in pre-exposed NHP that protect mice against lethal influenza challenge.[/h] van der Lubbe JEM[SUP]1[/SUP], Huizingh J[SUP]1[/SUP], Verspuij JWA[SUP]1[/SUP], Tettero L[SUP]1[/SUP], Schmit-Tillemans SPR[SUP]1[/SUP], Mooij P[SUP]2[/SUP], Mortier D[SUP]2[/SUP], Koopman G[SUP]2[/SUP], Bogers WMJM[SUP]2[/SUP], Dekking L[SUP]1[/SUP], Meijberg W[SUP]1,[/SUP][SUP]3[/SUP], Kwaks T[SUP]1[/SUP], Brandenburg B[SUP]1[/SUP], Tolboom JTBM[SUP]1[/SUP], Schuitemaker H[SUP]1[/SUP], Roozendaal R[SUP]1[/SUP], Kuipers H[SUP]#[/SUP][SUP]1[/SUP], Zahn RC[SUP]#[/SUP][SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Seasonal vaccines are currently the most effective countermeasure against influenza. However, seasonal vaccines are only effective against strains closely related to the influenza strains contained in the vaccine. Recently a new hemagglutinin (HA) stem-based antigen, the so-called "mini-HA", has been shown to induce a cross-protective immune response in influenza-naive mice and non-human primates (NHP). However, prior exposure to influenza can have a profound effect on the immune response to subsequent influenza infection and the protective efficacy of vaccination. Here we show that mini-HA, compared to a trivalent influenza vaccine (TIV), elicits a broadened influenza-specific humoral immune response in NHP previously exposed to influenza. Serum transfer experiments showed that antibodies induced by both mini-HA and seasonal vaccine protected mice against lethal challenge with a H1N1 influenza strain heterologous to the H1 HA included in the TIV. However, antibodies elicited by mini-HA showed an additional benefit of protecting mice against lethal heterosubtypic H5N1 influenza challenge, associated with H5 HA-specific functional antibodies.
PMID: 29977611 PMCID: PMC6030213 DOI: 10.1038/s41541-018-0063-7
Free PMC Article