tetano
Editor, Senior Moderator
Mikrochim Acta
. 2025 Feb 18;192(3):171.
doi: 10.1007/s00604-025-07031-1. Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA
Lingling Wang[SUP] 1 [/SUP], Shengjun Bu[SUP] 2 3 [/SUP], Heyuan Meng[SUP] 2 [/SUP], Xiaoying Zhang[SUP] 1 [/SUP], Hongyu Zhou[SUP] 2 [/SUP], Chuanna Xu[SUP] 3 [/SUP], Zhuo Hao[SUP] 2 [/SUP], Yuchun Su[SUP] 4 [/SUP], Jiayu Wan[SUP] 5 [/SUP]
Affiliations
A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10-10[SUP]6[/SUP] pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.
Keywords: Influenza virus; Isothermal amplification; Lateral flow assay; M13mp18 ssDNA.
. 2025 Feb 18;192(3):171.
doi: 10.1007/s00604-025-07031-1. Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA
Lingling Wang[SUP] 1 [/SUP], Shengjun Bu[SUP] 2 3 [/SUP], Heyuan Meng[SUP] 2 [/SUP], Xiaoying Zhang[SUP] 1 [/SUP], Hongyu Zhou[SUP] 2 [/SUP], Chuanna Xu[SUP] 3 [/SUP], Zhuo Hao[SUP] 2 [/SUP], Yuchun Su[SUP] 4 [/SUP], Jiayu Wan[SUP] 5 [/SUP]
Affiliations
- PMID: 39966254
- DOI: 10.1007/s00604-025-07031-1
A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10-10[SUP]6[/SUP] pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.
Keywords: Influenza virus; Isothermal amplification; Lateral flow assay; M13mp18 ssDNA.