tetano
Editor, Senior Moderator
Mikrochim Acta
. 2025 Nov 5;192(12):792.
doi: 10.1007/s00604-025-07544-9. CRISPR-assisted MoS[SUB]2[/SUB]@TQD nanosheets-based lateral flow assay for dual-mode and sensitive diagnosis of SARS-CoV-2
Yinuo Sun[SUP] 1 2 [/SUP], Han Han[SUP] 1 2 [/SUP], Rongtao Zhao[SUP] 3 [/SUP], Xiaoxian Liu[SUP] 1 [/SUP], Zhenzhen Liu[SUP] 1 [/SUP], Jing Liang[SUP] 1 [/SUP], Bingwei Wang[SUP] 1 [/SUP], Baohua Zhao[SUP] 4 [/SUP], Yuanfeng Pang[SUP] 5 [/SUP], Rui Xiao[SUP] 6 [/SUP]
Affiliations
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) assembled lateral flow assay (LFA) have been developed for rapid virus diagnosis, however, the single-mode visual half-quantitative characteristics limit their applications in accurate quantitative diagnosis. Herein, a CRISPR assisted MoS[SUB]2[/SUB]@TQD (MoS[SUB]2[/SUB] nanosheet assembled ternary quantum dot) based colorimetric and fluorescent dual-mode LFA was fabricated for the ultrasensitive and flexible detection of the SARS-CoV-2. Compared with single QDs, MoS[SUB]2[/SUB]@TQD can provide stable and superior colorimetric/fluorescent signals in various bio-samples. RT-RAA/CRISPR-Cas13 was adopted to couple with MoS[SUB]2[/SUB]@TQD so that the detection sensitivity can be further enhanced. Based on the dual-mode LFA, the colorimetric visual detection limit was 400 copies/mL, and for fluorescence quantification mode, as low as 250 copies/mL can be obtained. For clinic diagnosis, 35 SARS-CoV-2 positive throat swab samples and 18 negative samples were detected by this LFA, 100% accuracy and 100% consistency with quantitative polymerase chain reaction (qPCR) results can be obtained. It indicated that MoS[SUB]2[/SUB]@TQD-based dual-mode LFA can be a rapid, sensitive, and accurate detection platform for SARS-CoV-2 and other infectious pathogens in the point-of-care diagnosis.
Keywords: 2; CRISPR; Cas13; RT; CoV; MoS2@TQD;; Mode LFA; SARS; RAA.
. 2025 Nov 5;192(12):792.
doi: 10.1007/s00604-025-07544-9. CRISPR-assisted MoS[SUB]2[/SUB]@TQD nanosheets-based lateral flow assay for dual-mode and sensitive diagnosis of SARS-CoV-2
Yinuo Sun[SUP] 1 2 [/SUP], Han Han[SUP] 1 2 [/SUP], Rongtao Zhao[SUP] 3 [/SUP], Xiaoxian Liu[SUP] 1 [/SUP], Zhenzhen Liu[SUP] 1 [/SUP], Jing Liang[SUP] 1 [/SUP], Bingwei Wang[SUP] 1 [/SUP], Baohua Zhao[SUP] 4 [/SUP], Yuanfeng Pang[SUP] 5 [/SUP], Rui Xiao[SUP] 6 [/SUP]
Affiliations
- PMID: 41191093
- DOI: 10.1007/s00604-025-07544-9
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) assembled lateral flow assay (LFA) have been developed for rapid virus diagnosis, however, the single-mode visual half-quantitative characteristics limit their applications in accurate quantitative diagnosis. Herein, a CRISPR assisted MoS[SUB]2[/SUB]@TQD (MoS[SUB]2[/SUB] nanosheet assembled ternary quantum dot) based colorimetric and fluorescent dual-mode LFA was fabricated for the ultrasensitive and flexible detection of the SARS-CoV-2. Compared with single QDs, MoS[SUB]2[/SUB]@TQD can provide stable and superior colorimetric/fluorescent signals in various bio-samples. RT-RAA/CRISPR-Cas13 was adopted to couple with MoS[SUB]2[/SUB]@TQD so that the detection sensitivity can be further enhanced. Based on the dual-mode LFA, the colorimetric visual detection limit was 400 copies/mL, and for fluorescence quantification mode, as low as 250 copies/mL can be obtained. For clinic diagnosis, 35 SARS-CoV-2 positive throat swab samples and 18 negative samples were detected by this LFA, 100% accuracy and 100% consistency with quantitative polymerase chain reaction (qPCR) results can be obtained. It indicated that MoS[SUB]2[/SUB]@TQD-based dual-mode LFA can be a rapid, sensitive, and accurate detection platform for SARS-CoV-2 and other infectious pathogens in the point-of-care diagnosis.
Keywords: 2; CRISPR; Cas13; RT; CoV; MoS2@TQD;; Mode LFA; SARS; RAA.