tetano
Editor, Senior Moderator
Microorganisms
. 2022 Jan 10;10(1):133.
doi: 10.3390/microorganisms10010133.
Interferon Signaling in Chickens Plays a Crucial Role in Inhibiting Influenza Replication in DF1 Cells
Daniel S Layton[SUP] 1 [/SUP], Kostlend Mara[SUP] 1 [/SUP], Meiling Dai[SUP] 1 [/SUP], Luis Fernando Malaver-Ortega[SUP] 2 [/SUP], Tamara J Gough[SUP] 1 [/SUP], Kerri Bruce[SUP] 1 [/SUP], Kristie A Jenkins[SUP] 1 [/SUP], Andrew G D Bean[SUP] 1 [/SUP]
Affiliations
Abstract
Influenza A viruses (IAV) pose a constant threat to human and poultry health. Of particular interest are the infections caused by highly pathogenic avian influenza (HPAI) viruses, such as H5N1, which cause significant production issues. In response to influenza infection, cells activate immune mechanisms that lead to increased interferon (IFN) production. To investigate how alterations in the interferon signaling pathway affect the cellular response to infection in the chicken, we used CRISPR/Cas9 to generate a chicken cell line that lacks a functional the type I interferon receptor (IFNAR1). We then assessed viral infections with the WSN strain of influenza. Cells lacking a functional IFNAR1 receptor showed reduced expression of the interferon stimulated genes (ISG) such as Protein Kinase R (PKR) and Myxovirus resistance (Mx) and were more susceptible to viral infection with WSN. We further investigated the role or IFNAR1 on low pathogenicity avian influenza (LPAI) strains (H7N9) and a HPAI strain (H5N1). Intriguingly, Ifnar[SUP]-/-[/SUP] cells appeared more resistant than WT cells when infected with HPAI virus, potentially indicating a different interaction between H5N1 and the IFN signaling pathway. Our findings support that ChIFNAR1 is a key component of the chicken IFN signaling pathway and these data add contributions to the field of host-avian pathogen interaction and innate immunity in chickens.
Keywords: IFNAR; antiviral response; influenza virus; innate immunity; interferon stimulated genes; interferons; viral infection.
. 2022 Jan 10;10(1):133.
doi: 10.3390/microorganisms10010133.
Interferon Signaling in Chickens Plays a Crucial Role in Inhibiting Influenza Replication in DF1 Cells
Daniel S Layton[SUP] 1 [/SUP], Kostlend Mara[SUP] 1 [/SUP], Meiling Dai[SUP] 1 [/SUP], Luis Fernando Malaver-Ortega[SUP] 2 [/SUP], Tamara J Gough[SUP] 1 [/SUP], Kerri Bruce[SUP] 1 [/SUP], Kristie A Jenkins[SUP] 1 [/SUP], Andrew G D Bean[SUP] 1 [/SUP]
Affiliations
- PMID: 35056582
- DOI: 10.3390/microorganisms10010133
Abstract
Influenza A viruses (IAV) pose a constant threat to human and poultry health. Of particular interest are the infections caused by highly pathogenic avian influenza (HPAI) viruses, such as H5N1, which cause significant production issues. In response to influenza infection, cells activate immune mechanisms that lead to increased interferon (IFN) production. To investigate how alterations in the interferon signaling pathway affect the cellular response to infection in the chicken, we used CRISPR/Cas9 to generate a chicken cell line that lacks a functional the type I interferon receptor (IFNAR1). We then assessed viral infections with the WSN strain of influenza. Cells lacking a functional IFNAR1 receptor showed reduced expression of the interferon stimulated genes (ISG) such as Protein Kinase R (PKR) and Myxovirus resistance (Mx) and were more susceptible to viral infection with WSN. We further investigated the role or IFNAR1 on low pathogenicity avian influenza (LPAI) strains (H7N9) and a HPAI strain (H5N1). Intriguingly, Ifnar[SUP]-/-[/SUP] cells appeared more resistant than WT cells when infected with HPAI virus, potentially indicating a different interaction between H5N1 and the IFN signaling pathway. Our findings support that ChIFNAR1 is a key component of the chicken IFN signaling pathway and these data add contributions to the field of host-avian pathogen interaction and innate immunity in chickens.
Keywords: IFNAR; antiviral response; influenza virus; innate immunity; interferon stimulated genes; interferons; viral infection.