tetano
Editor, Senior Moderator
Microorganisms
. 2023 Jan 18;11(2):244.
doi: 10.3390/microorganisms11020244.
Genetic, Antigenic, and Pathobiological Characterization of H9 and H6 Low Pathogenicity Avian Influenza Viruses Isolated in Vietnam from 2014 to 2018
Kien Trung Le[SUP] 1 [/SUP], Lam Thanh Nguyen[SUP] 2 [/SUP], Loc Tan Huynh[SUP] 1 2 [/SUP], Duc-Huy Chu[SUP] 3 [/SUP], Long Van Nguyen[SUP] 3 [/SUP], Tien Ngoc Nguyen[SUP] 3 [/SUP], Tien Ngoc Tien[SUP] 4 [/SUP], Keita Matsuno[SUP] 1 5 6 [/SUP], Masatoshi Okamatsu[SUP] 1 [/SUP], Takahiro Hiono[SUP] 1 7 [/SUP], Norikazu Isoda[SUP] 1 6 7 [/SUP], Yoshihiro Sakoda[SUP] 1 6 7 8 [/SUP]
Affiliations
Abstract
The H9 and H6 subtypes of low pathogenicity avian influenza viruses (LPAIVs) cause substantial economic losses in poultry worldwide, including Vietnam. Herein, we characterized Vietnamese H9 and H6 LPAIVs to facilitate the control of avian influenza. The space-time representative viruses of each subtype were selected based on active surveillance from 2014 to 2018 in Vietnam. Phylogenetic analysis using hemagglutinin genes revealed that 54 H9 and 48 H6 Vietnamese LPAIVs were classified into the sublineages Y280/BJ94 and Group II, respectively. Gene constellation analysis indicated that 6 and 19 genotypes of the H9 and H6 subtypes, respectively, belonged to the representative viruses. The Vietnamese viruses are genetically related to the previous isolates and those in neighboring countries, indicating their circulation in poultry after being introduced into Vietnam. The antigenicity of these subtypes was different from that of viruses isolated from wild birds. Antigenicity was more conserved in the H9 viruses than in the H6 viruses. Furthermore, a representative H9 LPAIV exhibited systemic replication in chickens, which was enhanced by coinfection with avian pathogenic Escherichia coli O2. Although H9 and H6 were classified as LPAIVs, their characterization indicated that their silent spread might significantly affect the poultry industry.
Keywords: Escherichia coli O2; Vietnam; coinfection; low pathogenicity avian influenza; pathogenicity assessment; surveillance.
. 2023 Jan 18;11(2):244.
doi: 10.3390/microorganisms11020244.
Genetic, Antigenic, and Pathobiological Characterization of H9 and H6 Low Pathogenicity Avian Influenza Viruses Isolated in Vietnam from 2014 to 2018
Kien Trung Le[SUP] 1 [/SUP], Lam Thanh Nguyen[SUP] 2 [/SUP], Loc Tan Huynh[SUP] 1 2 [/SUP], Duc-Huy Chu[SUP] 3 [/SUP], Long Van Nguyen[SUP] 3 [/SUP], Tien Ngoc Nguyen[SUP] 3 [/SUP], Tien Ngoc Tien[SUP] 4 [/SUP], Keita Matsuno[SUP] 1 5 6 [/SUP], Masatoshi Okamatsu[SUP] 1 [/SUP], Takahiro Hiono[SUP] 1 7 [/SUP], Norikazu Isoda[SUP] 1 6 7 [/SUP], Yoshihiro Sakoda[SUP] 1 6 7 8 [/SUP]
Affiliations
- PMID: 36838209
- PMCID: PMC9962344
- DOI: 10.3390/microorganisms11020244
Abstract
The H9 and H6 subtypes of low pathogenicity avian influenza viruses (LPAIVs) cause substantial economic losses in poultry worldwide, including Vietnam. Herein, we characterized Vietnamese H9 and H6 LPAIVs to facilitate the control of avian influenza. The space-time representative viruses of each subtype were selected based on active surveillance from 2014 to 2018 in Vietnam. Phylogenetic analysis using hemagglutinin genes revealed that 54 H9 and 48 H6 Vietnamese LPAIVs were classified into the sublineages Y280/BJ94 and Group II, respectively. Gene constellation analysis indicated that 6 and 19 genotypes of the H9 and H6 subtypes, respectively, belonged to the representative viruses. The Vietnamese viruses are genetically related to the previous isolates and those in neighboring countries, indicating their circulation in poultry after being introduced into Vietnam. The antigenicity of these subtypes was different from that of viruses isolated from wild birds. Antigenicity was more conserved in the H9 viruses than in the H6 viruses. Furthermore, a representative H9 LPAIV exhibited systemic replication in chickens, which was enhanced by coinfection with avian pathogenic Escherichia coli O2. Although H9 and H6 were classified as LPAIVs, their characterization indicated that their silent spread might significantly affect the poultry industry.
Keywords: Escherichia coli O2; Vietnam; coinfection; low pathogenicity avian influenza; pathogenicity assessment; surveillance.