tetano
Editor, Senior Moderator
Sci Rep. 2017 Jul 18;7(1):5705. doi: 10.1038/s41598-017-05940-7.
[h=1]Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring.[/h] Esser ES[SUP]1[/SUP], Pulit-Penaloza JA[SUP]1[/SUP], Kalluri H[SUP]2[/SUP], McAllister D[SUP]2[/SUP], Vassilieva EV[SUP]1[/SUP], Littauer EQ[SUP]1[/SUP], Lelutiu N[SUP]1[/SUP], Prausnitz MR[SUP]2[/SUP], Compans RW[SUP]1[/SUP], Skountzou I[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus causes life-threatening infections in pregnant women and their newborns. Immunization during pregnancy is the most effective means of preventing maternal and infant mortality/morbidity; however, influenza vaccination rates of pregnant women remain under 50%. Furthermore, the availability of vaccines in low-resource populations is limited. Skin immunization with microneedle patches (MN) is a novel and safe vaccination platform featuring thermostable vaccine formulations. Cold-chain independence and the potential for self-administration can expand influenza vaccination coverage in developing countries. In this study of pregnant BALB/c mice immunized with subunit H1N1 influenza vaccine, we demonstrate the advantage of skin vaccination over intramuscular delivery of a two-fold higher vaccine dose. MN vaccine induced superior humoral immune responses and conferred protective immunity against a lethal challenge dose of homologous influenza virus. Importantly, MN vaccination of mice at mid-gestation resulted in enhanced and long-lasting passive immunity of the offspring, measured by neutralizing antibody titers and survival rates after virus challenge. We conclude that skin vaccination using MN is a superior immunization approach with the potential to overcome immune tolerance observed in pregnancy, and lower vaccination costs through antigen dose-sparing, which is especially relevant in underserved countries.
PMID: 28720851 PMCID: PMC5515933 DOI: 10.1038/s41598-017-05940-7
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[h=1]Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring.[/h] Esser ES[SUP]1[/SUP], Pulit-Penaloza JA[SUP]1[/SUP], Kalluri H[SUP]2[/SUP], McAllister D[SUP]2[/SUP], Vassilieva EV[SUP]1[/SUP], Littauer EQ[SUP]1[/SUP], Lelutiu N[SUP]1[/SUP], Prausnitz MR[SUP]2[/SUP], Compans RW[SUP]1[/SUP], Skountzou I[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus causes life-threatening infections in pregnant women and their newborns. Immunization during pregnancy is the most effective means of preventing maternal and infant mortality/morbidity; however, influenza vaccination rates of pregnant women remain under 50%. Furthermore, the availability of vaccines in low-resource populations is limited. Skin immunization with microneedle patches (MN) is a novel and safe vaccination platform featuring thermostable vaccine formulations. Cold-chain independence and the potential for self-administration can expand influenza vaccination coverage in developing countries. In this study of pregnant BALB/c mice immunized with subunit H1N1 influenza vaccine, we demonstrate the advantage of skin vaccination over intramuscular delivery of a two-fold higher vaccine dose. MN vaccine induced superior humoral immune responses and conferred protective immunity against a lethal challenge dose of homologous influenza virus. Importantly, MN vaccination of mice at mid-gestation resulted in enhanced and long-lasting passive immunity of the offspring, measured by neutralizing antibody titers and survival rates after virus challenge. We conclude that skin vaccination using MN is a superior immunization approach with the potential to overcome immune tolerance observed in pregnancy, and lower vaccination costs through antigen dose-sparing, which is especially relevant in underserved countries.
PMID: 28720851 PMCID: PMC5515933 DOI: 10.1038/s41598-017-05940-7
Free full text