tetano
Editor, Senior Moderator
Micromachines (Basel)
. 2021 Nov 27;12(12):1464.
doi: 10.3390/mi12121464.
Point-of-Care Testing-The Key in the Battle against SARS-CoV-2 Pandemic
Florina Silvia Iliescu[SUP] 1 [/SUP], Ana Maria Ionescu[SUP] 1 2 [/SUP], Larisa Gogianu[SUP] 1 [/SUP], Monica Simion[SUP] 1 [/SUP], Violeta Dediu[SUP] 1 [/SUP], Mariana Carmen Chifiriuc[SUP] 3 4 [/SUP], Gratiela Gradisteanu Pircalabioru[SUP] 3 [/SUP], Ciprian Iliescu[SUP] 1 5 6 [/SUP]
Affiliations
Abstract
The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the "gold standard" involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.
Keywords: SARS-CoV-2 variants; detection; epidemic; point of care testing.
. 2021 Nov 27;12(12):1464.
doi: 10.3390/mi12121464.
Point-of-Care Testing-The Key in the Battle against SARS-CoV-2 Pandemic
Florina Silvia Iliescu[SUP] 1 [/SUP], Ana Maria Ionescu[SUP] 1 2 [/SUP], Larisa Gogianu[SUP] 1 [/SUP], Monica Simion[SUP] 1 [/SUP], Violeta Dediu[SUP] 1 [/SUP], Mariana Carmen Chifiriuc[SUP] 3 4 [/SUP], Gratiela Gradisteanu Pircalabioru[SUP] 3 [/SUP], Ciprian Iliescu[SUP] 1 5 6 [/SUP]
Affiliations
- PMID: 34945314
- DOI: 10.3390/mi12121464
Abstract
The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the "gold standard" involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.
Keywords: SARS-CoV-2 variants; detection; epidemic; point of care testing.