tetano
Editor, Senior Moderator
Microchem J
. 2021 Dec 1;107046.
doi: 10.1016/j.microc.2021.107046. Online ahead of print.
Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle
Nan Li[SUP] 1 [/SUP], Linhong Shi[SUP] 1 [/SUP], Xue Zou[SUP] 1 [/SUP], Tengfei Wang[SUP] 1 [/SUP], Dongmei Wang[SUP] 1 2 [/SUP], Zhengjun Gong[SUP] 1 2 [/SUP], Meikun Fan[SUP] 1 2 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has afflicted the world, is a highly contagious virus. The timely and accurate diagnosis of SARS-CoV-2 infections is vital for disease control and prevention. This work is that a fluorescence immunoassay was developed to detect 2019-nCoV antibodies (2019-nCoV mAb). Fluorescent graphene quantum dots (GQDs) and Ag@Au nanoparticles (Ag@AuNPs) were successfully synthesized and characterized. Fluorescence resonance energy transfer (FRET) enables Ag@AuNPs effective quenching of GQDs fluorescence.. With the presence of 2019-nCoV mAb, a steric hindrance was observed between the Ag@AuNPs-NCP (2019-nCoV antigen) complex and GQDs, which reduced the FRET efficiency and restored the fluorescence of GQDs. The fluorescence enhancement efficiency has a satisfactory linear relationship with the logarithm of the 2019-nCoV mAb in a concentration scope of 0.1 pg mL[SUP]-1[/SUP] - 10 ng mL[SUP]-1[/SUP], and the limit of detection was 50 fg mL[SUP]-1[/SUP]. This study tested other proteins and proved that the fluorescence immunoassay has good selectivity. When this method was applied to the standard addition recovery trial test of 2019-nCoV mAb in serum samples, the recovery rate was between 90.8% and 103.3%, and satisfactory results were obtained.
Keywords: 2019-nCoV; Ag@AuNPs; Fluorescence immunosensor; Fluorescence resonance energy transfer; Graphene quantum dots.
. 2021 Dec 1;107046.
doi: 10.1016/j.microc.2021.107046. Online ahead of print.
Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle
Nan Li[SUP] 1 [/SUP], Linhong Shi[SUP] 1 [/SUP], Xue Zou[SUP] 1 [/SUP], Tengfei Wang[SUP] 1 [/SUP], Dongmei Wang[SUP] 1 2 [/SUP], Zhengjun Gong[SUP] 1 2 [/SUP], Meikun Fan[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34866656
- PMCID: PMC8632743
- DOI: 10.1016/j.microc.2021.107046
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has afflicted the world, is a highly contagious virus. The timely and accurate diagnosis of SARS-CoV-2 infections is vital for disease control and prevention. This work is that a fluorescence immunoassay was developed to detect 2019-nCoV antibodies (2019-nCoV mAb). Fluorescent graphene quantum dots (GQDs) and Ag@Au nanoparticles (Ag@AuNPs) were successfully synthesized and characterized. Fluorescence resonance energy transfer (FRET) enables Ag@AuNPs effective quenching of GQDs fluorescence.. With the presence of 2019-nCoV mAb, a steric hindrance was observed between the Ag@AuNPs-NCP (2019-nCoV antigen) complex and GQDs, which reduced the FRET efficiency and restored the fluorescence of GQDs. The fluorescence enhancement efficiency has a satisfactory linear relationship with the logarithm of the 2019-nCoV mAb in a concentration scope of 0.1 pg mL[SUP]-1[/SUP] - 10 ng mL[SUP]-1[/SUP], and the limit of detection was 50 fg mL[SUP]-1[/SUP]. This study tested other proteins and proved that the fluorescence immunoassay has good selectivity. When this method was applied to the standard addition recovery trial test of 2019-nCoV mAb in serum samples, the recovery rate was between 90.8% and 103.3%, and satisfactory results were obtained.
Keywords: 2019-nCoV; Ag@AuNPs; Fluorescence immunosensor; Fluorescence resonance energy transfer; Graphene quantum dots.