tetano
Editor, Senior Moderator
Microbiol Spectr
. 2021 Nov 17;e0101721.
doi: 10.1128/Spectrum.01017-21. Online ahead of print.
CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern
Yuanhao Liang[SUP] 1 [/SUP], Hongqing Lin[SUP] 1 [/SUP], Lirong Zou[SUP] 2 [/SUP], Jianhui Zhao[SUP] 1 [/SUP], Baisheng Li[SUP] 2 [/SUP], Haiying Wang[SUP] 1 [/SUP], Jing Lu[SUP] 2 [/SUP], Jiufeng Sun[SUP] 2 [/SUP], Xingfen Yang[SUP] 1 [/SUP], Xiaoling Deng[SUP] 2 [/SUP], Shixing Tang[SUP] 1 3 4 [/SUP]
Affiliations
Abstract
A big challenge for the control of COVID-19 pandemic is the emergence of variants of concern (VOCs) or variants of interest (VOIs) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which may be more transmissible and/or more virulent and could escape immunity obtained through infection or vaccination. A simple and rapid test for SARS-CoV-2 variants is an unmet need and is of great public health importance. In this study, we designed and analytically validated a CRISPR-Cas12a system for direct detection of SARS-CoV-2 VOCs. We further evaluated the combination of ordinary reverse transcription-PCR (RT-PCR) and CRISPR-Cas12a to improve the detection sensitivity and developed a universal system by introducing a protospacer adjacent motif (PAM) near the target mutation sites through PCR primer design to detect mutations without PAM. Our results indicated that the CRISPR-Cas12a assay could readily detect the signature spike protein mutations (K417N/T, L452R/Q, T478K, E484K/Q, N501Y, and D614G) to distinguish alpha, beta, gamma, delta, kappa, lambda, and epsilon variants of SARS-CoV-2. In addition, the open reading frame 8 (ORF8) mutations (T/C substitution at nt28144 and the corresponding change of amino acid L/S) could differentiate L and S lineages of SARS-CoV-2. The low limit of detection could reach 10 copies/reaction. Our assay successfully distinguished 4 SARS-CoV-2 strains of wild type and alpha (B.1.1.7), beta (B.1.351), and delta (B.1.617.2) variants. By testing 32 SARS-CoV-2-positive clinical samples infected with the wild type (n = 5) and alpha (n = 11), beta (n = 8), and delta variants (n = 8), the concordance between our assay and sequencing was 100%. The CRISPR-based approach is rapid and robust and can be adapted for screening the emerging mutations and immediately implemented in laboratories already performing nucleic acid amplification tests or in resource-limited settings. IMPORTANCE We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented for screening of SARS-CoV-2 variants in resource-limited settings. This approach can be adapted for emerging mutations and implemented in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests using existing resources and extracted nucleic acid.
Keywords: CRISPR-Cas system; SARS-CoV-2; emerging SARS-CoV-2 variant; variant screening.
. 2021 Nov 17;e0101721.
doi: 10.1128/Spectrum.01017-21. Online ahead of print.
CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern
Yuanhao Liang[SUP] 1 [/SUP], Hongqing Lin[SUP] 1 [/SUP], Lirong Zou[SUP] 2 [/SUP], Jianhui Zhao[SUP] 1 [/SUP], Baisheng Li[SUP] 2 [/SUP], Haiying Wang[SUP] 1 [/SUP], Jing Lu[SUP] 2 [/SUP], Jiufeng Sun[SUP] 2 [/SUP], Xingfen Yang[SUP] 1 [/SUP], Xiaoling Deng[SUP] 2 [/SUP], Shixing Tang[SUP] 1 3 4 [/SUP]
Affiliations
- PMID: 34787487
- DOI: 10.1128/Spectrum.01017-21
Abstract
A big challenge for the control of COVID-19 pandemic is the emergence of variants of concern (VOCs) or variants of interest (VOIs) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which may be more transmissible and/or more virulent and could escape immunity obtained through infection or vaccination. A simple and rapid test for SARS-CoV-2 variants is an unmet need and is of great public health importance. In this study, we designed and analytically validated a CRISPR-Cas12a system for direct detection of SARS-CoV-2 VOCs. We further evaluated the combination of ordinary reverse transcription-PCR (RT-PCR) and CRISPR-Cas12a to improve the detection sensitivity and developed a universal system by introducing a protospacer adjacent motif (PAM) near the target mutation sites through PCR primer design to detect mutations without PAM. Our results indicated that the CRISPR-Cas12a assay could readily detect the signature spike protein mutations (K417N/T, L452R/Q, T478K, E484K/Q, N501Y, and D614G) to distinguish alpha, beta, gamma, delta, kappa, lambda, and epsilon variants of SARS-CoV-2. In addition, the open reading frame 8 (ORF8) mutations (T/C substitution at nt28144 and the corresponding change of amino acid L/S) could differentiate L and S lineages of SARS-CoV-2. The low limit of detection could reach 10 copies/reaction. Our assay successfully distinguished 4 SARS-CoV-2 strains of wild type and alpha (B.1.1.7), beta (B.1.351), and delta (B.1.617.2) variants. By testing 32 SARS-CoV-2-positive clinical samples infected with the wild type (n = 5) and alpha (n = 11), beta (n = 8), and delta variants (n = 8), the concordance between our assay and sequencing was 100%. The CRISPR-based approach is rapid and robust and can be adapted for screening the emerging mutations and immediately implemented in laboratories already performing nucleic acid amplification tests or in resource-limited settings. IMPORTANCE We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented for screening of SARS-CoV-2 variants in resource-limited settings. This approach can be adapted for emerging mutations and implemented in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests using existing resources and extracted nucleic acid.
Keywords: CRISPR-Cas system; SARS-CoV-2; emerging SARS-CoV-2 variant; variant screening.