tetano
Editor, Senior Moderator
Microbes Infect
. 2024 Mar 25:105331.
doi: 10.1016/j.micinf.2024.105331. Online ahead of print. Virome survey of the bat, Rhinolophus affinis, in Hainan Province, China
Zihan Li[SUP] 1 [/SUP], Chuanning Tang[SUP] 2 [/SUP], Youyou Li[SUP] 3 [/SUP], Yun Zhang[SUP] 2 [/SUP], Gaoyu Wang[SUP] 2 [/SUP], Ruoyan Peng[SUP] 2 [/SUP], Yi Huang[SUP] 2 [/SUP], Xiaoyuan Hu[SUP] 2 [/SUP], Henan Xin[SUP] 4 [/SUP], Xuefang Cao[SUP] 4 [/SUP], Lingyu Shen[SUP] 4 [/SUP], Tonglei Guo[SUP] 4 [/SUP], Yijun He[SUP] 4 [/SUP], Boxuan Fen[SUP] 4 [/SUP], Juanjuan Huang[SUP] 4 [/SUP], Jian Guo Liang[SUP] 4 [/SUP], Xiuji Cui[SUP] 2 [/SUP], Lina Niu[SUP] 2 [/SUP], Jian Yang[SUP] 4 [/SUP], Fan Yang[SUP] 4 [/SUP], Gang Lu[SUP] 2 [/SUP], Lei Gao[SUP] 4 [/SUP], Qi Jin[SUP] 4 [/SUP], Mingming Zhao[SUP] 5 [/SUP], Feifei Yin[SUP] 6 [/SUP], Jiang Du[SUP] 7 [/SUP]
Affiliations
Bats are important mammal reservoirs of zoonotic pathogens. However, due to research limitations involving species, locations, pathogens, or sample types, the full diversity of viruses in bats remains to be discovered. We used next-generation sequencing technology to characterize the mammalian virome and analyze the phylogenetic evolution and diversity of mammalian viruses carried by bats from Haikou City and Tunchang County in Hainan Province, China. We collected 200 pharyngeal swab and anal swab samples from Rhinolophus affinis, combining them into nine pools based on the sample type and collection location. We subjected the samples to next-generation sequencing and conducted bioinformatics analysis. All samples were screened via specific PCR and phylogenetic analysis. The diverse viral reads, closely related to mammals, were assigned into 17 viral families. We discovered many novel bat viruses and identified some closely related to known human/animal pathogens. In the current study, 6 complete genomes and 2 partial genomic sequences of 6 viral families and 8 viral genera have been amplified, among which 5 strains are suggested to be new virus species. These included coronavirus, pestivirus, bastrovirus, bocavirus, papillomavirus, parvovirus, and paramyxovirus. The primary finding is that a SADS-related CoV and a HoBi-like pestivirus identified in Rhinolophus affinis in Hainan Province could be pathogenic to livestock. This study expands our understanding of bats as a virus reservoir, providing a basis for further research on the transmission of viruses from bats to humans.
Keywords: Rhinolophus affinis; Viral diversity; evolution; zoonosis.
. 2024 Mar 25:105331.
doi: 10.1016/j.micinf.2024.105331. Online ahead of print. Virome survey of the bat, Rhinolophus affinis, in Hainan Province, China
Zihan Li[SUP] 1 [/SUP], Chuanning Tang[SUP] 2 [/SUP], Youyou Li[SUP] 3 [/SUP], Yun Zhang[SUP] 2 [/SUP], Gaoyu Wang[SUP] 2 [/SUP], Ruoyan Peng[SUP] 2 [/SUP], Yi Huang[SUP] 2 [/SUP], Xiaoyuan Hu[SUP] 2 [/SUP], Henan Xin[SUP] 4 [/SUP], Xuefang Cao[SUP] 4 [/SUP], Lingyu Shen[SUP] 4 [/SUP], Tonglei Guo[SUP] 4 [/SUP], Yijun He[SUP] 4 [/SUP], Boxuan Fen[SUP] 4 [/SUP], Juanjuan Huang[SUP] 4 [/SUP], Jian Guo Liang[SUP] 4 [/SUP], Xiuji Cui[SUP] 2 [/SUP], Lina Niu[SUP] 2 [/SUP], Jian Yang[SUP] 4 [/SUP], Fan Yang[SUP] 4 [/SUP], Gang Lu[SUP] 2 [/SUP], Lei Gao[SUP] 4 [/SUP], Qi Jin[SUP] 4 [/SUP], Mingming Zhao[SUP] 5 [/SUP], Feifei Yin[SUP] 6 [/SUP], Jiang Du[SUP] 7 [/SUP]
Affiliations
- PMID: 38537769
- DOI: 10.1016/j.micinf.2024.105331
Bats are important mammal reservoirs of zoonotic pathogens. However, due to research limitations involving species, locations, pathogens, or sample types, the full diversity of viruses in bats remains to be discovered. We used next-generation sequencing technology to characterize the mammalian virome and analyze the phylogenetic evolution and diversity of mammalian viruses carried by bats from Haikou City and Tunchang County in Hainan Province, China. We collected 200 pharyngeal swab and anal swab samples from Rhinolophus affinis, combining them into nine pools based on the sample type and collection location. We subjected the samples to next-generation sequencing and conducted bioinformatics analysis. All samples were screened via specific PCR and phylogenetic analysis. The diverse viral reads, closely related to mammals, were assigned into 17 viral families. We discovered many novel bat viruses and identified some closely related to known human/animal pathogens. In the current study, 6 complete genomes and 2 partial genomic sequences of 6 viral families and 8 viral genera have been amplified, among which 5 strains are suggested to be new virus species. These included coronavirus, pestivirus, bastrovirus, bocavirus, papillomavirus, parvovirus, and paramyxovirus. The primary finding is that a SADS-related CoV and a HoBi-like pestivirus identified in Rhinolophus affinis in Hainan Province could be pathogenic to livestock. This study expands our understanding of bats as a virus reservoir, providing a basis for further research on the transmission of viruses from bats to humans.
Keywords: Rhinolophus affinis; Viral diversity; evolution; zoonosis.