tetano
Editor, Senior Moderator
Microb Cell Fact
. 2020 Oct 15;19(1):193.
doi: 10.1186/s12934-020-01453-7.
Haemagglutinin displayed on the surface of Lactococcus lactis confers broad cross-clade protection against different H5N1 viruses in chickens
Han Lei[SUP] 1 [/SUP], Tong Gao[SUP] 2 [/SUP], Qianhong Cen[SUP] 2 [/SUP], Xiaojue Peng[SUP] 3 [/SUP]
Affiliations
Abstract
Background: The highly pathogenic avian influenza (HPAI) H5N1 virus poses a potential threat to the poultry industry. The currently available avian influenza H5N1 vaccines for poultry are clade-specific. Therefore, an effective vaccine for preventing and controlling H5N1 viruses belonging to different clades needs to be developed.
Results: Recombinant L. lactis/pNZ8148-Spax-HA was generated, and the influenza virus haemagglutinin (HA) protein of A/Vietnam/1203/2004 (H5N1) was displayed on the surface of Lactococcus lactis (L. lactis). Spax was used as an anchor protein. Chickens vaccinated orally with unadjuvanted L. lactis/pNZ8148-Spax-HA could produce significant humoral and mucosal responses and neutralizing activities against H5N1 viruses belonging to different clades. Importantly, unadjuvanted L. lactis/pNZ8148-Spax-HA conferred cross-clade protection against lethal challenge with different H5N1 viruses in the chicken model.
Conclusion: This study provides insights into the cross-clade protection conferred by unadjuvanted L. lactis/pNZ8148-Spax-HA, and the results might help the establishment of a promising platform for the development of a safe and effective H5N1 cross-clade vaccine for poultry.
Keywords: Cross protection; H5N1 cross-clade vaccine; L. lactis/pNZ8148-spax-HA.
. 2020 Oct 15;19(1):193.
doi: 10.1186/s12934-020-01453-7.
Haemagglutinin displayed on the surface of Lactococcus lactis confers broad cross-clade protection against different H5N1 viruses in chickens
Han Lei[SUP] 1 [/SUP], Tong Gao[SUP] 2 [/SUP], Qianhong Cen[SUP] 2 [/SUP], Xiaojue Peng[SUP] 3 [/SUP]
Affiliations
- PMID: 33059676
- DOI: 10.1186/s12934-020-01453-7
Abstract
Background: The highly pathogenic avian influenza (HPAI) H5N1 virus poses a potential threat to the poultry industry. The currently available avian influenza H5N1 vaccines for poultry are clade-specific. Therefore, an effective vaccine for preventing and controlling H5N1 viruses belonging to different clades needs to be developed.
Results: Recombinant L. lactis/pNZ8148-Spax-HA was generated, and the influenza virus haemagglutinin (HA) protein of A/Vietnam/1203/2004 (H5N1) was displayed on the surface of Lactococcus lactis (L. lactis). Spax was used as an anchor protein. Chickens vaccinated orally with unadjuvanted L. lactis/pNZ8148-Spax-HA could produce significant humoral and mucosal responses and neutralizing activities against H5N1 viruses belonging to different clades. Importantly, unadjuvanted L. lactis/pNZ8148-Spax-HA conferred cross-clade protection against lethal challenge with different H5N1 viruses in the chicken model.
Conclusion: This study provides insights into the cross-clade protection conferred by unadjuvanted L. lactis/pNZ8148-Spax-HA, and the results might help the establishment of a promising platform for the development of a safe and effective H5N1 cross-clade vaccine for poultry.
Keywords: Cross protection; H5N1 cross-clade vaccine; L. lactis/pNZ8148-spax-HA.