tetano
Editor, Senior Moderator
Methods Mol Biol
. 2022;2380:201-209.
doi: 10.1007/978-1-0716-1736-6_17.
Evaluation of Human Circulating T Follicular Helper Cells in Influenza- and SARS-CoV-2-Specific B Cell Immunity
Marios Koutsakos[SUP] 1 [/SUP], Katherine Kedzierska[SUP] #[/SUP][SUP] 2 3 [/SUP], Thi H O Nguyen[SUP] #[/SUP][SUP] 4 [/SUP]
Affiliations
Abstract
Generation of effective immune protection against viral infection and vaccination depends greatly on a successful engagement and stimulation of adaptive immune B cells and a specialized CD4[SUP]+[/SUP] T cell subset called T follicular helper cells (T[SUB]FH[/SUB] cells). Since T[SUB]FH[/SUB] cells primarily reside in lymphoid tissues, they can be challenging to study in human settings. However, a counterpart of these cells, circulating T[SUB]FH[/SUB] (cT[SUB]FH[/SUB]) cells, can be detected in peripheral blood. Assessment of cT[SUB]FH[/SUB] cells serves as an informative marker of humoral responses following viral infection and vaccination and can be predictive of antibody titers. Here, we describe a comprehensive flow cytometry detection method for dissecting cT[SUB]FH[/SUB] subsets and activation, together with the assessment of antibody-secreting cells (ASCs), from a small volume of human whole blood. This approach allows the investigation of cellular events that underpin successful immune responses following influenza and SARS-CoV-2 infection/vaccination in humans and is applicable to other viral disease settings.
Keywords: COVID-19; Circulating T follicular helper cells; Human blood; Influenza.
. 2022;2380:201-209.
doi: 10.1007/978-1-0716-1736-6_17.
Evaluation of Human Circulating T Follicular Helper Cells in Influenza- and SARS-CoV-2-Specific B Cell Immunity
Marios Koutsakos[SUP] 1 [/SUP], Katherine Kedzierska[SUP] #[/SUP][SUP] 2 3 [/SUP], Thi H O Nguyen[SUP] #[/SUP][SUP] 4 [/SUP]
Affiliations
- PMID: 34802133
- DOI: 10.1007/978-1-0716-1736-6_17
Abstract
Generation of effective immune protection against viral infection and vaccination depends greatly on a successful engagement and stimulation of adaptive immune B cells and a specialized CD4[SUP]+[/SUP] T cell subset called T follicular helper cells (T[SUB]FH[/SUB] cells). Since T[SUB]FH[/SUB] cells primarily reside in lymphoid tissues, they can be challenging to study in human settings. However, a counterpart of these cells, circulating T[SUB]FH[/SUB] (cT[SUB]FH[/SUB]) cells, can be detected in peripheral blood. Assessment of cT[SUB]FH[/SUB] cells serves as an informative marker of humoral responses following viral infection and vaccination and can be predictive of antibody titers. Here, we describe a comprehensive flow cytometry detection method for dissecting cT[SUB]FH[/SUB] subsets and activation, together with the assessment of antibody-secreting cells (ASCs), from a small volume of human whole blood. This approach allows the investigation of cellular events that underpin successful immune responses following influenza and SARS-CoV-2 infection/vaccination in humans and is applicable to other viral disease settings.
Keywords: COVID-19; Circulating T follicular helper cells; Human blood; Influenza.