tetano
Editor, Senior Moderator
Am J Physiol Regul Integr Comp Physiol. 2016 Aug 24:ajpregu.00298.2016. doi: 10.1152/ajpregu.00298.2016. [Epub ahead of print]
[h=1]Metabolic pathways of lung inflammation revealed by high-resolution metabolomics (HRM) of H1N1 influenza virus infection in mice.[/h] Chandler JD[SUP]1[/SUP], Hu X[SUP]1[/SUP], Ko EJ, Park S, Lee YT, Orr ML[SUP]1[/SUP], Fernandes J, Uppal K, Kang SM[SUP]2[/SUP], Jones DP[SUP]1[/SUP], Go YM[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a significant health concern worldwide. Viral infection induces local and systemic activation of the immune system causing attendant changes in metabolism. High-resolution metabolomics (HRM) uses advanced mass spectrometry and computational methods to measure thousands of metabolites inclusive of most metabolic pathways. We used HRM to identify metabolic pathways and clusters of association related to inflammatory cytokines in lungs of mice with H1N1 influenza virus infection. Infected mice showed progressive weight loss, decreased lung function and severe lung inflammation with elevated cytokines [interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ] and increased oxidative stress via cysteine oxidation. HRM showed prominent effects of influenza virus infection on tryptophan and other amino acids, and widespread effects on pathways including purines, pyrimidines, fatty acids and glycerophospholipids. A metabolome-wide association study (MWAS) of the aforementioned inflammatory cytokines was used to determine the relationship of metabolic responses to inflammation during infection. This cytokine-MWAS (cMWAS) showed that metabolic associations consisted of distinct and shared clusters of 396 metabolites highly correlated with inflammatory cytokines. Strong negative associations of selected glycerosphingolipid, linoleate and tryptophan metabolites with IFN-γ contrasted strong positive associations of glycosphingolipid and bile acid metabolites with IL-1β, TNF-α and IL-10. Anti-inflammatory cytokine IL-10 had strong positive associations with vitamin D3, purine and vitamin E metabolism. The detailed metabolic interactions with cytokines indicate that targeted metabolic interventions may be useful during life-threatening crises related to severe acute infection and inflammation.
Copyright ? 2016, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology.
[h=4]KEYWORDS:[/h] Metabolic pathway analysis; mouse lung metabolome; pulmonary disease; targeted metabolic intervention
PMID: 27558316 DOI: 10.1152/ajpregu.00298.2016
[PubMed - as supplied by publisher]
[h=1]Metabolic pathways of lung inflammation revealed by high-resolution metabolomics (HRM) of H1N1 influenza virus infection in mice.[/h] Chandler JD[SUP]1[/SUP], Hu X[SUP]1[/SUP], Ko EJ, Park S, Lee YT, Orr ML[SUP]1[/SUP], Fernandes J, Uppal K, Kang SM[SUP]2[/SUP], Jones DP[SUP]1[/SUP], Go YM[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a significant health concern worldwide. Viral infection induces local and systemic activation of the immune system causing attendant changes in metabolism. High-resolution metabolomics (HRM) uses advanced mass spectrometry and computational methods to measure thousands of metabolites inclusive of most metabolic pathways. We used HRM to identify metabolic pathways and clusters of association related to inflammatory cytokines in lungs of mice with H1N1 influenza virus infection. Infected mice showed progressive weight loss, decreased lung function and severe lung inflammation with elevated cytokines [interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ] and increased oxidative stress via cysteine oxidation. HRM showed prominent effects of influenza virus infection on tryptophan and other amino acids, and widespread effects on pathways including purines, pyrimidines, fatty acids and glycerophospholipids. A metabolome-wide association study (MWAS) of the aforementioned inflammatory cytokines was used to determine the relationship of metabolic responses to inflammation during infection. This cytokine-MWAS (cMWAS) showed that metabolic associations consisted of distinct and shared clusters of 396 metabolites highly correlated with inflammatory cytokines. Strong negative associations of selected glycerosphingolipid, linoleate and tryptophan metabolites with IFN-γ contrasted strong positive associations of glycosphingolipid and bile acid metabolites with IL-1β, TNF-α and IL-10. Anti-inflammatory cytokine IL-10 had strong positive associations with vitamin D3, purine and vitamin E metabolism. The detailed metabolic interactions with cytokines indicate that targeted metabolic interventions may be useful during life-threatening crises related to severe acute infection and inflammation.
Copyright ? 2016, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology.
[h=4]KEYWORDS:[/h] Metabolic pathway analysis; mouse lung metabolome; pulmonary disease; targeted metabolic intervention
PMID: 27558316 DOI: 10.1152/ajpregu.00298.2016
[PubMed - as supplied by publisher]