tetano
Editor, Senior Moderator
Med Microbiol Immunol
. 2022 Jan 21.
doi: 10.1007/s00430-022-00728-7. Online ahead of print.
Rapid and sensitive identification of omicron by variant-specific PCR and nanopore sequencing: paradigm for diagnostics of emerging SARS-CoV-2 variants
Christopher Dächert[SUP] #[/SUP][SUP] 1 [/SUP], Maximilian Muenchhoff[SUP] #[/SUP][SUP] 1 2 [/SUP], Alexander Graf[SUP] #[/SUP][SUP] 1 3 [/SUP], Hanna Autenrieth[SUP] 1 [/SUP], Sabine Bender[SUP] 1 [/SUP], Helga Mairhofer[SUP] 1 [/SUP], Paul R Wratil[SUP] 1 [/SUP], Susanne Thieme[SUP] 3 [/SUP], Stefan Krebs[SUP] 3 [/SUP], Natascha Grzimek-Koschewa[SUP] 1 2 [/SUP], Helmut Blum[SUP] 3 [/SUP], Oliver T Keppler[SUP] 4 [/SUP]
Affiliations
Abstract
On November 26, 2021, the World Health Organization classified B.1.1.529 as a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern (VoC), named omicron. Spike-gene dropouts in conventional SARS-CoV-2 PCR systems have been reported over the last weeks as indirect diagnostic evidence for the identification of omicron. Here, we report the combination of PCRs specific for heavily mutated sites in the spike gene and nanopore-based full-length genome sequencing for the rapid and sensitive identification of the first four COVID-19 patients diagnosed in Germany to be infected with omicron on November 28, 2021. This study will assist the unambiguous laboratory-based diagnosis and global surveillance for this highly contagious VoC with an unprecedented degree of humoral immune escape. Moreover, we propose that specialized diagnostic laboratories should continuously update their assays for variant-specific PCRs in the spike gene of SARS-CoV-2 to readily detect and diagnose emerging variants of interest and VoCs. The combination with established nanopore sequencing procedures allows both the rapid confirmation by whole genome sequencing as well as the sensitive identification of newly emerging variants of this pandemic β-coronavirus in years to come.
Keywords: Melting curve analysis; Nanopore; Omicron; SARS-CoV-2; VOC; VOC-PCR.
. 2022 Jan 21.
doi: 10.1007/s00430-022-00728-7. Online ahead of print.
Rapid and sensitive identification of omicron by variant-specific PCR and nanopore sequencing: paradigm for diagnostics of emerging SARS-CoV-2 variants
Christopher Dächert[SUP] #[/SUP][SUP] 1 [/SUP], Maximilian Muenchhoff[SUP] #[/SUP][SUP] 1 2 [/SUP], Alexander Graf[SUP] #[/SUP][SUP] 1 3 [/SUP], Hanna Autenrieth[SUP] 1 [/SUP], Sabine Bender[SUP] 1 [/SUP], Helga Mairhofer[SUP] 1 [/SUP], Paul R Wratil[SUP] 1 [/SUP], Susanne Thieme[SUP] 3 [/SUP], Stefan Krebs[SUP] 3 [/SUP], Natascha Grzimek-Koschewa[SUP] 1 2 [/SUP], Helmut Blum[SUP] 3 [/SUP], Oliver T Keppler[SUP] 4 [/SUP]
Affiliations
- PMID: 35061086
- DOI: 10.1007/s00430-022-00728-7
Abstract
On November 26, 2021, the World Health Organization classified B.1.1.529 as a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern (VoC), named omicron. Spike-gene dropouts in conventional SARS-CoV-2 PCR systems have been reported over the last weeks as indirect diagnostic evidence for the identification of omicron. Here, we report the combination of PCRs specific for heavily mutated sites in the spike gene and nanopore-based full-length genome sequencing for the rapid and sensitive identification of the first four COVID-19 patients diagnosed in Germany to be infected with omicron on November 28, 2021. This study will assist the unambiguous laboratory-based diagnosis and global surveillance for this highly contagious VoC with an unprecedented degree of humoral immune escape. Moreover, we propose that specialized diagnostic laboratories should continuously update their assays for variant-specific PCRs in the spike gene of SARS-CoV-2 to readily detect and diagnose emerging variants of interest and VoCs. The combination with established nanopore sequencing procedures allows both the rapid confirmation by whole genome sequencing as well as the sensitive identification of newly emerging variants of this pandemic β-coronavirus in years to come.
Keywords: Melting curve analysis; Nanopore; Omicron; SARS-CoV-2; VOC; VOC-PCR.