tetano
Editor, Senior Moderator
Med Microbiol Immunol
. 2024 May 9;213(1):6.
doi: 10.1007/s00430-024-00789-w. Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
Helena Müller-Kräuter[SUP] #[/SUP][SUP] 1 [/SUP], Jolanda Mezzacapo[SUP] #[/SUP][SUP] 1 [/SUP], Michael Klüver[SUP] 1 2 [/SUP], Sara Baumgart[SUP] 1 [/SUP], Dirk Becker[SUP] 1 2 [/SUP], Anahita Fathi[SUP] 3 4 [/SUP], Sebastian Pfeiffer[SUP] 1 [/SUP], Verena Krähling[SUP] 5 6 [/SUP]
Affiliations
To date, there is no licensed vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV). Therefore, MERS-CoV is one of the diseases targeted by the Coalition for Epidemic Preparedness Innovations (CEPI) vaccine development programs and has been classified as a priority disease by the World Health Organization (WHO). An important measure of vaccine immunogenicity and antibody functionality is the detection of virus-neutralizing antibodies. We have developed and optimized a microneutralization assay (MNA) using authentic MERS-CoV and standardized automatic counting of virus foci. Compared to our standard virus neutralization assay, the MNA showed improved sensitivity when analyzing 30 human sera with good correlation of results (Spearman's correlation coefficient r = 0.8917, p value < 0.0001). It is important to use standardized materials, such as the WHO international standard (IS) for anti-MERS-CoV immunoglobulin G, to compare the results from clinical trials worldwide. Therefore, in addition to the neutralizing titers (NT[SUB]50[/SUB] = 1384, NT[SUB]80[/SUB] = 384), we determined the IC[SUB]50[/SUB] and IC[SUB]80[/SUB] of WHO IS in our MNA to be 0.67 IU/ml and 2.6 IU/ml, respectively. Overall, the established MNA is well suited to reliably quantify vaccine-induced neutralizing antibodies with high sensitivity.
Keywords: MERS-CoV; MNA; Middle East respiratory syndrome coronavirus; Neutralization; VNT; Vaccine.
. 2024 May 9;213(1):6.
doi: 10.1007/s00430-024-00789-w. Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
Helena Müller-Kräuter[SUP] #[/SUP][SUP] 1 [/SUP], Jolanda Mezzacapo[SUP] #[/SUP][SUP] 1 [/SUP], Michael Klüver[SUP] 1 2 [/SUP], Sara Baumgart[SUP] 1 [/SUP], Dirk Becker[SUP] 1 2 [/SUP], Anahita Fathi[SUP] 3 4 [/SUP], Sebastian Pfeiffer[SUP] 1 [/SUP], Verena Krähling[SUP] 5 6 [/SUP]
Affiliations
- PMID: 38722338
- DOI: 10.1007/s00430-024-00789-w
To date, there is no licensed vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV). Therefore, MERS-CoV is one of the diseases targeted by the Coalition for Epidemic Preparedness Innovations (CEPI) vaccine development programs and has been classified as a priority disease by the World Health Organization (WHO). An important measure of vaccine immunogenicity and antibody functionality is the detection of virus-neutralizing antibodies. We have developed and optimized a microneutralization assay (MNA) using authentic MERS-CoV and standardized automatic counting of virus foci. Compared to our standard virus neutralization assay, the MNA showed improved sensitivity when analyzing 30 human sera with good correlation of results (Spearman's correlation coefficient r = 0.8917, p value < 0.0001). It is important to use standardized materials, such as the WHO international standard (IS) for anti-MERS-CoV immunoglobulin G, to compare the results from clinical trials worldwide. Therefore, in addition to the neutralizing titers (NT[SUB]50[/SUB] = 1384, NT[SUB]80[/SUB] = 384), we determined the IC[SUB]50[/SUB] and IC[SUB]80[/SUB] of WHO IS in our MNA to be 0.67 IU/ml and 2.6 IU/ml, respectively. Overall, the established MNA is well suited to reliably quantify vaccine-induced neutralizing antibodies with high sensitivity.
Keywords: MERS-CoV; MNA; Middle East respiratory syndrome coronavirus; Neutralization; VNT; Vaccine.