Giuseppe
Emeritus
Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria (mBio, abstract, edited)
[Source: mBio, full text: <cite cite="http://mbio.asm.org/content/1/4/e00208-10.short?rss=1">Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria ? mBio</cite>. Abstract, edited.]
Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
1. Phenix-Lan Quan a, 2. Cadhla Firth a, 3. Craig Street a, 4. Jose A. Henriquez a, 5. Alexandra Petrosov a, 6. Alla Tashmukhamedova a, 7. Stephen K. Hutchison b, 8. Michael Egholm b, 9. Modupe O. V. Osinubi c, 10. Michael Niezgoda c, 11. Albert B. Ogunkoya d, 12. Thomas Briese a, 13. Charles E. Rupprecht c, and 14. W. Ian Lipkin a
Author Affiliations
1. Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USAa;
2. 454 Life Sciences, Branford, Connecticut, USAb;
3. Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USAc; and
4. Department of Veterinary Surgery and Medicine, Ahmadu Bello University, Zaria, Nigeriad
1. Address correspondence to Phenix-Lan Quan, pq2106@columbia.edu.
1. Editor Anne Moscona, Weill Cornell Medical College
ABSTRACT
Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson?s leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.
IMPORTANCE
Bats (order Chiroptera, suborders Megachiroptera and Microchiroptera) are reservoirs for a wide range of viruses that cause diseases in humans and livestock, including the severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for the global SARS outbreak in 2003. The diversity of viruses harbored by bats is only just beginning to be understood because of expanded wildlife surveillance and the development and application of new tools for pathogen discovery. This paper describes a new coronavirus, one with a distinctive genomic organization that may provide insights into coronavirus evolution and biology.
Footnotes
* Citation Quan, P.-L., C. Firth, C. Street, J. A. Henriquez, A. Petrosov, et al. 2010. Identification of a severe acute respiratory syndrome coronavirus-like virus in a leaf-nosed bat in Nigeria. mBio 1(4):e00208-10. doi:10.1128/mBio.00208-10.
* Received 25 August 2010
* Accepted 3 September 2010
* Published 12 October 2010
* Copyright ? 2010 Quan et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
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[Source: mBio, full text: <cite cite="http://mbio.asm.org/content/1/4/e00208-10.short?rss=1">Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria ? mBio</cite>. Abstract, edited.]
Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
1. Phenix-Lan Quan a, 2. Cadhla Firth a, 3. Craig Street a, 4. Jose A. Henriquez a, 5. Alexandra Petrosov a, 6. Alla Tashmukhamedova a, 7. Stephen K. Hutchison b, 8. Michael Egholm b, 9. Modupe O. V. Osinubi c, 10. Michael Niezgoda c, 11. Albert B. Ogunkoya d, 12. Thomas Briese a, 13. Charles E. Rupprecht c, and 14. W. Ian Lipkin a
Author Affiliations
1. Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USAa;
2. 454 Life Sciences, Branford, Connecticut, USAb;
3. Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USAc; and
4. Department of Veterinary Surgery and Medicine, Ahmadu Bello University, Zaria, Nigeriad
1. Address correspondence to Phenix-Lan Quan, pq2106@columbia.edu.
1. Editor Anne Moscona, Weill Cornell Medical College
ABSTRACT
Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson?s leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.
IMPORTANCE
Bats (order Chiroptera, suborders Megachiroptera and Microchiroptera) are reservoirs for a wide range of viruses that cause diseases in humans and livestock, including the severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for the global SARS outbreak in 2003. The diversity of viruses harbored by bats is only just beginning to be understood because of expanded wildlife surveillance and the development and application of new tools for pathogen discovery. This paper describes a new coronavirus, one with a distinctive genomic organization that may provide insights into coronavirus evolution and biology.
Footnotes
* Citation Quan, P.-L., C. Firth, C. Street, J. A. Henriquez, A. Petrosov, et al. 2010. Identification of a severe acute respiratory syndrome coronavirus-like virus in a leaf-nosed bat in Nigeria. mBio 1(4):e00208-10. doi:10.1128/mBio.00208-10.
* Received 25 August 2010
* Accepted 3 September 2010
* Published 12 October 2010
* Copyright ? 2010 Quan et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
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