tetano
Editor, Senior Moderator
mBio
. 2026 Nov 3:e0157426.
doi: 10.1128/mbio.01574-26. Online ahead of print.
Hannah L Fischer 1 2 , Shanmugapriya Shanmugapriya 1 , Christopher Kline 1 , W Paul Duprex 1 2 , Kevin R McCarthy 1 2 , Simon C Watkins 3 , James F Conway 4 , Zandrea Ambrose 1 2
Affiliations Expand
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a highly transmissible acute respiratory infection that can result in severe pneumonia and death. Many details of SARS-CoV-2 infection are not fully understood, including the cell biology and host-virus interactions involved in coronavirus assembly and release, in which the Envelope (E) structural protein is instrumental. Deletion of E in other coronaviruses has been shown previously to either attenuate or abrogate infection. To determine the role of E in SARS-CoV-2 virus production and infectivity, we produced a reporter SARS-CoV-2 with or without the E gene deleted using a bacterial artificial chromosome. Replication of ΔE SARS-CoV-2 was attenuated in Vero E6 cells expressing human ACE2 and TMPRSS2 and in human epithelial cell lines. Electron and immunofluorescence microscopy and virology assays showed that ΔE SARS-CoV-2 increased cell surface expression of Spike (S) glycoprotein, leading to reduced S incorporation into ΔE SARS-CoV-2 particles and promotion of increased cell-to-cell transmission that evades neutralizing antibody inhibition. Trans-complementation of E partially rescued ΔE SARS-CoV-2 S incorporation and restored cell-free transmission. In addition to validating the role of E in retention of S in the ER-Golgi intermediate complex (ERGIC), our results showed that a lack of E led to reorganization of the ERGIC and Golgi during SARS-CoV-2 infection. Improved understanding of E in SARS-CoV-2 replication and host pathogenesis may help the development of novel therapeutics.IMPORTANCENon-Spike coronavirus structural proteins, including Envelope, are conserved, making them potential pan-coronavirus therapeutic targets. Many details about these proteins and their roles in viral replication and host pathogenesis are unknown. In this study, we showed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates without Envelope but is attenuated and impaired in virus particle formation, with less Spike incorporated into virions and more Spike expressed on the cell surface compared to wild-type virus. SARS-CoV-2 lacking Envelope transmitted primarily via cell fusion and evaded neutralizing antibodies. In addition, the absence of Envelope resulted in the reorganization of the cell secretory compartment during SARS-CoV-2 infection. A better understanding of how Envelope influences SARS-CoV-2 replication could guide directed design of novel therapeutics for treatment of COVID-19 patients, as well as the potential for pan-coronavirus protection against future coronavirus outbreaks.
Keywords: Envelope (E); Membrane (M); SARS-CoV-2; transmission; virus assembly.
. 2026 Nov 3:e0157426.
doi: 10.1128/mbio.01574-26. Online ahead of print.
Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered spike localization and increased cell-to-cell transmission
Hannah L Fischer 1 2 , Shanmugapriya Shanmugapriya 1 , Christopher Kline 1 , W Paul Duprex 1 2 , Kevin R McCarthy 1 2 , Simon C Watkins 3 , James F Conway 4 , Zandrea Ambrose 1 2
Affiliations Expand
- PMID: 42975141
- DOI: 10.1128/mbio.01574-26
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a highly transmissible acute respiratory infection that can result in severe pneumonia and death. Many details of SARS-CoV-2 infection are not fully understood, including the cell biology and host-virus interactions involved in coronavirus assembly and release, in which the Envelope (E) structural protein is instrumental. Deletion of E in other coronaviruses has been shown previously to either attenuate or abrogate infection. To determine the role of E in SARS-CoV-2 virus production and infectivity, we produced a reporter SARS-CoV-2 with or without the E gene deleted using a bacterial artificial chromosome. Replication of ΔE SARS-CoV-2 was attenuated in Vero E6 cells expressing human ACE2 and TMPRSS2 and in human epithelial cell lines. Electron and immunofluorescence microscopy and virology assays showed that ΔE SARS-CoV-2 increased cell surface expression of Spike (S) glycoprotein, leading to reduced S incorporation into ΔE SARS-CoV-2 particles and promotion of increased cell-to-cell transmission that evades neutralizing antibody inhibition. Trans-complementation of E partially rescued ΔE SARS-CoV-2 S incorporation and restored cell-free transmission. In addition to validating the role of E in retention of S in the ER-Golgi intermediate complex (ERGIC), our results showed that a lack of E led to reorganization of the ERGIC and Golgi during SARS-CoV-2 infection. Improved understanding of E in SARS-CoV-2 replication and host pathogenesis may help the development of novel therapeutics.IMPORTANCENon-Spike coronavirus structural proteins, including Envelope, are conserved, making them potential pan-coronavirus therapeutic targets. Many details about these proteins and their roles in viral replication and host pathogenesis are unknown. In this study, we showed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates without Envelope but is attenuated and impaired in virus particle formation, with less Spike incorporated into virions and more Spike expressed on the cell surface compared to wild-type virus. SARS-CoV-2 lacking Envelope transmitted primarily via cell fusion and evaded neutralizing antibodies. In addition, the absence of Envelope resulted in the reorganization of the cell secretory compartment during SARS-CoV-2 infection. A better understanding of how Envelope influences SARS-CoV-2 replication could guide directed design of novel therapeutics for treatment of COVID-19 patients, as well as the potential for pan-coronavirus protection against future coronavirus outbreaks.
Keywords: Envelope (E); Membrane (M); SARS-CoV-2; transmission; virus assembly.