tetano
Editor, Senior Moderator
Mater Today Chem
. 2022 Apr 22;100924.
doi: 10.1016/j.mtchem.2022.100924. Online ahead of print.
Influence of particle size on the SARS-CoV-2 spike protein detection using IgG-capped gold nanoparticles and dynamic light scattering (DLS)
Carolina B P Ligiero[SUP] 1 [/SUP], Tamires S Fernandes[SUP] 1 [/SUP], Dayenny L D'Amato[SUP] 1 [/SUP], Francisco V Gaspar[SUP] 1 2 [/SUP], Paola S Duarte[SUP] 2 [/SUP], Marcelo Abrahão Strauch[SUP] 3 [/SUP], Juliana G Fonseca[SUP] 3 [/SUP], Leonardo G R Meirelles[SUP] 4 [/SUP], Patrícia Bento da Silva[SUP] 5 [/SUP], Ricardo B Azevedo[SUP] 5 [/SUP], Glêndara Aparecida de Souza Martins[SUP] 6 [/SUP], Bráulio S Archanjo[SUP] 7 [/SUP], Camilla D Buarque[SUP] 2 [/SUP], Giovanna Machado[SUP] 8 [/SUP], Ana M Percebom[SUP] 2 [/SUP], Célia Machado Ronconi[SUP] 1 [/SUP]
Affiliations
Abstract
Due to the unprecedented and ongoing nature of the coronavirus outbreak, the development of rapid immunoassays to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its highly contagious variants is an important and challenging task. Here, we report the development of polyclonal antibody-functionalized spherical gold nanoparticle biosensors (pAb-AuNPs) as well as the influence of the nanoparticle sizes on the immunoassay response to detect the SARS-CoV-2 spike protein (S protein) by dynamic light scattering (DLS). By monitoring the increment in the hydrodynamic diameter (ΔD[SUB]H[/SUB]) by DLS measurements in the antigen-antibody interaction, SARS-CoV-2 S-protein can be detected in only 5 minutes. The larger the nanoparticles, the larger ΔD[SUB]H[/SUB] in the presence of S protein. From adsorption isotherm, the calculated binding constant (K [SUB]D[/SUB] ) was 83 nM and the estimated limit of detection was 13 ng mL[SUP]-1[/SUP] (30 pM). The biosensor was stable up to 90 days at 4 °C. Therefore, the biosensor developed in this work could be potentially applied as a fast and sensible immunoassay to detect SARS-CoV-2 infection in patient samples.
Keywords: Dynamic Light Scattering; Gold nanoparticles Size Effect; Immunoassay; SARS-CoV-2 Spike Protein.
. 2022 Apr 22;100924.
doi: 10.1016/j.mtchem.2022.100924. Online ahead of print.
Influence of particle size on the SARS-CoV-2 spike protein detection using IgG-capped gold nanoparticles and dynamic light scattering (DLS)
Carolina B P Ligiero[SUP] 1 [/SUP], Tamires S Fernandes[SUP] 1 [/SUP], Dayenny L D'Amato[SUP] 1 [/SUP], Francisco V Gaspar[SUP] 1 2 [/SUP], Paola S Duarte[SUP] 2 [/SUP], Marcelo Abrahão Strauch[SUP] 3 [/SUP], Juliana G Fonseca[SUP] 3 [/SUP], Leonardo G R Meirelles[SUP] 4 [/SUP], Patrícia Bento da Silva[SUP] 5 [/SUP], Ricardo B Azevedo[SUP] 5 [/SUP], Glêndara Aparecida de Souza Martins[SUP] 6 [/SUP], Bráulio S Archanjo[SUP] 7 [/SUP], Camilla D Buarque[SUP] 2 [/SUP], Giovanna Machado[SUP] 8 [/SUP], Ana M Percebom[SUP] 2 [/SUP], Célia Machado Ronconi[SUP] 1 [/SUP]
Affiliations
- PMID: 35475288
- PMCID: PMC9023328
- DOI: 10.1016/j.mtchem.2022.100924
Abstract
Due to the unprecedented and ongoing nature of the coronavirus outbreak, the development of rapid immunoassays to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its highly contagious variants is an important and challenging task. Here, we report the development of polyclonal antibody-functionalized spherical gold nanoparticle biosensors (pAb-AuNPs) as well as the influence of the nanoparticle sizes on the immunoassay response to detect the SARS-CoV-2 spike protein (S protein) by dynamic light scattering (DLS). By monitoring the increment in the hydrodynamic diameter (ΔD[SUB]H[/SUB]) by DLS measurements in the antigen-antibody interaction, SARS-CoV-2 S-protein can be detected in only 5 minutes. The larger the nanoparticles, the larger ΔD[SUB]H[/SUB] in the presence of S protein. From adsorption isotherm, the calculated binding constant (K [SUB]D[/SUB] ) was 83 nM and the estimated limit of detection was 13 ng mL[SUP]-1[/SUP] (30 pM). The biosensor was stable up to 90 days at 4 °C. Therefore, the biosensor developed in this work could be potentially applied as a fast and sensible immunoassay to detect SARS-CoV-2 infection in patient samples.
Keywords: Dynamic Light Scattering; Gold nanoparticles Size Effect; Immunoassay; SARS-CoV-2 Spike Protein.