tetano
Editor, Senior Moderator
Environ Health Prev Med. 2010 Nov;15(6):344-9. Epub 2010 May 12.
Maintenance of influenza virus infectivity on the surfaces of personal protective equipment and clothing used in healthcare settings.
Sakaguchi H, Wada K, Kajioka J, Watanabe M, Nakano R, Hirose T, Ohta H, Aizawa Y.
Department of Occupational Health, Graduate School of Medical Sciences, Kitasato University, Tokyo, Japan.
Abstract
OBJECTIVES: The maintenance of infectivity of influenza viruses on the surfaces of personal protective equipment and clothing is an important factor in terms of controlling viral cross-infection in the environment and preventing contact infection. The aim of this study was to determine if laboratory-grown influenza A (H1N1) virus maintained infectivity on the surfaces of personal protective equipment and clothing used in healthcare settings.
METHODS: Influenza A virus (0.5 mL) was deposited on the surface of a rubber glove, an N95 particulate respirator, a surgical mask made of non-woven fabric, a gown made of Dupont Tyvek, a coated wooden desk, and stainless steel. Each sample was left for 1, 8, and 24 h, and hemagglutination (HA) and 50% tissue culture infective dose (TCID(50))/mL were measured.
RESULTS: The HA titer of this influenza A virus did not decrease in any of the materials tested even after 24 h. The infectivity of influenza A virus measured by TCID(50) was maintained for 8 h on the surface of all materials, with the exception of the rubber glove for which virus infectivity was maintained for 24 h.
CONCLUSIONS: Our results indicate that the replacement/renewal of personal protective equipment and clothing by healthcare professionals in cases of exposure to secretions and droplets containing viruses spread by patients is an appropriate procedure to prevent cross-infection.
PMID: 21432565 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21432565
Maintenance of influenza virus infectivity on the surfaces of personal protective equipment and clothing used in healthcare settings.
Sakaguchi H, Wada K, Kajioka J, Watanabe M, Nakano R, Hirose T, Ohta H, Aizawa Y.
Department of Occupational Health, Graduate School of Medical Sciences, Kitasato University, Tokyo, Japan.
Abstract
OBJECTIVES: The maintenance of infectivity of influenza viruses on the surfaces of personal protective equipment and clothing is an important factor in terms of controlling viral cross-infection in the environment and preventing contact infection. The aim of this study was to determine if laboratory-grown influenza A (H1N1) virus maintained infectivity on the surfaces of personal protective equipment and clothing used in healthcare settings.
METHODS: Influenza A virus (0.5 mL) was deposited on the surface of a rubber glove, an N95 particulate respirator, a surgical mask made of non-woven fabric, a gown made of Dupont Tyvek, a coated wooden desk, and stainless steel. Each sample was left for 1, 8, and 24 h, and hemagglutination (HA) and 50% tissue culture infective dose (TCID(50))/mL were measured.
RESULTS: The HA titer of this influenza A virus did not decrease in any of the materials tested even after 24 h. The infectivity of influenza A virus measured by TCID(50) was maintained for 8 h on the surface of all materials, with the exception of the rubber glove for which virus infectivity was maintained for 24 h.
CONCLUSIONS: Our results indicate that the replacement/renewal of personal protective equipment and clothing by healthcare professionals in cases of exposure to secretions and droplets containing viruses spread by patients is an appropriate procedure to prevent cross-infection.
PMID: 21432565 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21432565