tetano
Editor, Senior Moderator
Nanomedicine. 2013 Dec 9. pii: S1549-9634(13)00674-6. doi: 10.1016/j.nano.2013.11.009. [Epub ahead of print]
Magnetic Nanoparticle-based Immunoassay for Rapid Detection of Influenza Infections by Using an Integrated Microfluidic System.
Hung LY1, Chang JC2, Tsai YC3, Huang CC2, Chang CP3, Yeh CS4, Lee GB5.
Author information
Abstract
Magnetic manganese ferrite (MnFe2O4) nanoparticles with approximately 100nm diameter were used to improve the performance of an immunoassay for detecting influenza infections. The synthesized nanoparticles were tested for long-term storage to confirm the stability of their thermal decomposition process. Then, an integrated microfluidic system was developed to perform the diagnosis process automatically, including virus purification and detection. To apply these nanoparticles for influenza diagnosis, a micromixer was optimized to reduce the dead volume within the microfluidic chip. Furthermore, the mixing index of the micromixer could achieve as high as 97% in 2seconds. The optical signals showed that this nanoparticle-based immunoassay with dynamic mixing could successfully achieve a detection limit of influenza as low as 0.007 HAU. When compared with the 4.5-μm magnetic beads, the optical signals of the MnFe2O4 nanoparticles were twice as sensitive. Furthermore, five clinical specimens were tested to verify the usability of the developed system.
Copyright ? 2013. Published by Elsevier Inc.
KEYWORDS:
BSA, C(+), CCD, D(+), DI, DMEM, Dulbecco?s modified Eagle?s medium, EDC, ELISA, EMVs, FIA, HAU, JCPDS, Joint Committee on Powder Diffraction Standards, LBL, LOC, MDCK, MEMS, Mardin-Darby canine kidney cells, Micro-electro-mechanical-systems, N-(3-Dimethylaminopropyl)-N-ethyl carbodiimide hydrochloride, One-way ANOVA, P123, PAA, PAH, PBS, PCR, PDMS, PE, PEI, PMT, R-phycoerythrin, RB, SARS, TEM, TPCK, UV, X-ray diffractometer, XRD, anti-influenza-A-nucleoprotein, bovine serum albumin, charge-coupled device, deionized, electromagnetic valves, enzyme-linked immunosorbent assay, fluorescence immunoassay, hemagglutin units, influenza, lab-on-a-chip, layer-by-layer, mAbs, microfluidics, monoclonal antibodies, nanoparticles, normalized concentration, normalized location across the micromixer, one-way analysis of variance, phosphate-buffered saline, photo-multiplier tube, poly(acrylic acid), poly(dimethylsiloxane), poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer, poly(ethyleneimine)), polyallylamine hydrochloride, polymerase chain reaction, round bottom, severe acute respiratory syndrome, surface modification, transmission electron microscope, type XIII l-1-tosylamide-2-phenylethyl chloromethyl ketone, ultraviolet, α-A-NP
PMID:
24333595
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24333595
Magnetic Nanoparticle-based Immunoassay for Rapid Detection of Influenza Infections by Using an Integrated Microfluidic System.
Hung LY1, Chang JC2, Tsai YC3, Huang CC2, Chang CP3, Yeh CS4, Lee GB5.
Author information
Abstract
Magnetic manganese ferrite (MnFe2O4) nanoparticles with approximately 100nm diameter were used to improve the performance of an immunoassay for detecting influenza infections. The synthesized nanoparticles were tested for long-term storage to confirm the stability of their thermal decomposition process. Then, an integrated microfluidic system was developed to perform the diagnosis process automatically, including virus purification and detection. To apply these nanoparticles for influenza diagnosis, a micromixer was optimized to reduce the dead volume within the microfluidic chip. Furthermore, the mixing index of the micromixer could achieve as high as 97% in 2seconds. The optical signals showed that this nanoparticle-based immunoassay with dynamic mixing could successfully achieve a detection limit of influenza as low as 0.007 HAU. When compared with the 4.5-μm magnetic beads, the optical signals of the MnFe2O4 nanoparticles were twice as sensitive. Furthermore, five clinical specimens were tested to verify the usability of the developed system.
Copyright ? 2013. Published by Elsevier Inc.
KEYWORDS:
BSA, C(+), CCD, D(+), DI, DMEM, Dulbecco?s modified Eagle?s medium, EDC, ELISA, EMVs, FIA, HAU, JCPDS, Joint Committee on Powder Diffraction Standards, LBL, LOC, MDCK, MEMS, Mardin-Darby canine kidney cells, Micro-electro-mechanical-systems, N-(3-Dimethylaminopropyl)-N-ethyl carbodiimide hydrochloride, One-way ANOVA, P123, PAA, PAH, PBS, PCR, PDMS, PE, PEI, PMT, R-phycoerythrin, RB, SARS, TEM, TPCK, UV, X-ray diffractometer, XRD, anti-influenza-A-nucleoprotein, bovine serum albumin, charge-coupled device, deionized, electromagnetic valves, enzyme-linked immunosorbent assay, fluorescence immunoassay, hemagglutin units, influenza, lab-on-a-chip, layer-by-layer, mAbs, microfluidics, monoclonal antibodies, nanoparticles, normalized concentration, normalized location across the micromixer, one-way analysis of variance, phosphate-buffered saline, photo-multiplier tube, poly(acrylic acid), poly(dimethylsiloxane), poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer, poly(ethyleneimine)), polyallylamine hydrochloride, polymerase chain reaction, round bottom, severe acute respiratory syndrome, surface modification, transmission electron microscope, type XIII l-1-tosylamide-2-phenylethyl chloromethyl ketone, ultraviolet, α-A-NP
PMID:
24333595
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24333595