tetano
Editor, Senior Moderator
Mol Biol Evol. 2019 Nov 21. pii: msz276. doi: 10.1093/molbev/msz276. [Epub ahead of print] [h=1]Machine Learning Methods for Predicting Human-adaptative Influenza A Viruses Based on Viral Nucleotide Compositions.[/h]
Li J[SUP]1[/SUP], Zhang S[SUP]1[/SUP], Li B[SUP]2[/SUP], Hu Y[SUP]1[/SUP], Kang XP[SUP]1[/SUP], Wu XY[SUP]1[/SUP], Huang MT[SUP]1,[/SUP][SUP]3[/SUP], Li YC[SUP]1[/SUP], Zhao ZP[SUP]4[/SUP], Qin CF[SUP]1[/SUP], Jiang T[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h] 1 Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. 2 Department of Clinical Laboratory, the Fifth Medical Centre of Chinese PLA General Hospital, Beijing, China. 3 Graduate School, Anhui Medical University, Hefei, China. 4 Department of Infection and Immunology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
[h=3]Abstract[/h] Each influenza pandemic was caused at least partly by avian- and/or swine-origin influenza A viruses (IAVs). The timing of and the potential IAVs involved in the next pandemic are currently unpredictable. We aim to build machine learning (ML) models to predict human-adaptative IAV nucleotide composition. 217,549 IAV full-length coding sequences of the PB2 (Polymerase basic protein-2), PB1, PA (Polymerase acidic protein), HA (Hemagglutinin), NP (Nucleoprotein), NA (Neuraminidase) segments were decomposed for their codon position-based mononucleotides (12 nts) and dinucleotides (48 dnts). 68,742 human sequences and 68,739 avian sequences (1:1) were resampled to characterize the human adaptation-associated (d)nts with principal component analysis (PCA) and other ML models. Then, the human adaptation of IAV sequences was predicted based on the characterized (d)nts. Respectively, 9, 12, 11, 13 and 10 human-adaptive (d)nts were optimized for the six segments. PCA and hierarchical clustering analysis revealed the linear separability of the optimized (d)nts between the human-adaptive and avian-adaptive sets. The results of the confusion matrix and the area under the receiver operating characteristic (ROC) curve (AUC) indicated a high performance of the ML models to predict human adaptation of IAVs. Our model performed well in predicting the human adaptation of the swine/avian IAVs before and after the 2009 H1N1 pandemic. In conclusion, we identified the human adaptation-associated genomic composition of IAV segments. ML models for IAV human adaptation prediction using large IAV genomic datasets can facilitate the identification of key viral factors that affect virus transmission/pathogenicity. Most importantly, it allows the prediction of pandemic influenza.
? The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
[h=4]KEYWORDS:[/h] Dinucleotide; Genomic nucleotide composition; Human adaptation; Influenza A viruses (IAVs); Machine learning (ML)
PMID: 31750915 DOI: 10.1093/molbev/msz276
Li J[SUP]1[/SUP], Zhang S[SUP]1[/SUP], Li B[SUP]2[/SUP], Hu Y[SUP]1[/SUP], Kang XP[SUP]1[/SUP], Wu XY[SUP]1[/SUP], Huang MT[SUP]1,[/SUP][SUP]3[/SUP], Li YC[SUP]1[/SUP], Zhao ZP[SUP]4[/SUP], Qin CF[SUP]1[/SUP], Jiang T[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h] 1 Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. 2 Department of Clinical Laboratory, the Fifth Medical Centre of Chinese PLA General Hospital, Beijing, China. 3 Graduate School, Anhui Medical University, Hefei, China. 4 Department of Infection and Immunology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
[h=3]Abstract[/h] Each influenza pandemic was caused at least partly by avian- and/or swine-origin influenza A viruses (IAVs). The timing of and the potential IAVs involved in the next pandemic are currently unpredictable. We aim to build machine learning (ML) models to predict human-adaptative IAV nucleotide composition. 217,549 IAV full-length coding sequences of the PB2 (Polymerase basic protein-2), PB1, PA (Polymerase acidic protein), HA (Hemagglutinin), NP (Nucleoprotein), NA (Neuraminidase) segments were decomposed for their codon position-based mononucleotides (12 nts) and dinucleotides (48 dnts). 68,742 human sequences and 68,739 avian sequences (1:1) were resampled to characterize the human adaptation-associated (d)nts with principal component analysis (PCA) and other ML models. Then, the human adaptation of IAV sequences was predicted based on the characterized (d)nts. Respectively, 9, 12, 11, 13 and 10 human-adaptive (d)nts were optimized for the six segments. PCA and hierarchical clustering analysis revealed the linear separability of the optimized (d)nts between the human-adaptive and avian-adaptive sets. The results of the confusion matrix and the area under the receiver operating characteristic (ROC) curve (AUC) indicated a high performance of the ML models to predict human adaptation of IAVs. Our model performed well in predicting the human adaptation of the swine/avian IAVs before and after the 2009 H1N1 pandemic. In conclusion, we identified the human adaptation-associated genomic composition of IAV segments. ML models for IAV human adaptation prediction using large IAV genomic datasets can facilitate the identification of key viral factors that affect virus transmission/pathogenicity. Most importantly, it allows the prediction of pandemic influenza.
? The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
[h=4]KEYWORDS:[/h] Dinucleotide; Genomic nucleotide composition; Human adaptation; Influenza A viruses (IAVs); Machine learning (ML)
PMID: 31750915 DOI: 10.1093/molbev/msz276