tetano
Editor, Senior Moderator
J Infect Dis. 2018 Jan 24. doi: 10.1093/infdis/jiy035. [Epub ahead of print]
[h=1]Lung-derived exosomal miR-483-3p regulates the innate immune response to influenza virus infection.[/h] Maemura T[SUP]1,[/SUP][SUP]2[/SUP], Fukuyama S[SUP]1[/SUP], Sugita Y[SUP]3[/SUP], Lopes TJS[SUP]4[/SUP], Nakao T[SUP]1[/SUP], Noda T[SUP]5[/SUP], Kawaoka Y[SUP]1,[/SUP][SUP]4,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Exosomes regulate cell-cell communication by transferring functional proteins and RNAs between cells. Here, to clarify the function of exosomes during influenza virus infection, we characterized lung-derived exosomal microRNAs (miRNAs). Among the detected miRNAs, miR-483-3p was present at high levels in bronchoalveolar lavage fluid (BALF) exosomes during infection of mice with various strains of influenza virus, and miR-483-3p transfection potentiated gene expression of type I interferon and proinflammatory cytokine upon viral infection of MLE-12 cells. RNF5, a regulator of the RIG-I signaling pathway, was identified as a target gene of miR-483-3p. Moreover, we found that CD81, another miR-483-3p target, functions as a negative regulator of RIG-I signaling in MLE-12 cells. Taken together, this study indicates that BALF exosomal miRNAs may mediate the antiviral and inflammatory response to influenza virus infection.
[h=4]KEYWORDS:[/h] exosome; influenza virus; innate immunity; microRNA
PMID: 29373693 DOI: 10.1093/infdis/jiy035
[h=1]Lung-derived exosomal miR-483-3p regulates the innate immune response to influenza virus infection.[/h] Maemura T[SUP]1,[/SUP][SUP]2[/SUP], Fukuyama S[SUP]1[/SUP], Sugita Y[SUP]3[/SUP], Lopes TJS[SUP]4[/SUP], Nakao T[SUP]1[/SUP], Noda T[SUP]5[/SUP], Kawaoka Y[SUP]1,[/SUP][SUP]4,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Exosomes regulate cell-cell communication by transferring functional proteins and RNAs between cells. Here, to clarify the function of exosomes during influenza virus infection, we characterized lung-derived exosomal microRNAs (miRNAs). Among the detected miRNAs, miR-483-3p was present at high levels in bronchoalveolar lavage fluid (BALF) exosomes during infection of mice with various strains of influenza virus, and miR-483-3p transfection potentiated gene expression of type I interferon and proinflammatory cytokine upon viral infection of MLE-12 cells. RNF5, a regulator of the RIG-I signaling pathway, was identified as a target gene of miR-483-3p. Moreover, we found that CD81, another miR-483-3p target, functions as a negative regulator of RIG-I signaling in MLE-12 cells. Taken together, this study indicates that BALF exosomal miRNAs may mediate the antiviral and inflammatory response to influenza virus infection.
[h=4]KEYWORDS:[/h] exosome; influenza virus; innate immunity; microRNA
PMID: 29373693 DOI: 10.1093/infdis/jiy035