• FluTrackers.com Inc. does not provide medical advice. Information on this web site is collected from various internet resources, and the FluTrackers board of directors makes no warranty to the safety, efficacy, correctness or completeness of the information posted on this site by any author or poster. The information collated here is for instructional and/or discussion purposes only and is NOT intended to diagnose or treat any disease, illness, or other medical condition. Every individual reader or poster should seek advice from their personal physician/healthcare practitioner before considering or using any interventions that are discussed on this website. By continuing to access this website you agree to consult your personal physican before using any interventions posted on this website, and you agree to hold harmless FluTrackers.com Inc., the board of directors, the members, and all authors and posters for any effects from use of any medication, supplement, vitamin or other substance, device, intervention, etc. mentioned in posts on this website, or other internet venues referenced in posts on this website.
  • We are not asking for any donations. Do not donate to any entity who says they are raising funds for us.

Low Path H5-N1 in Montana ducks

Re: Low Path H5-N1 in Montana ducks

Snowy Owl said:
Canada is free of such events probably because of our bilingual signs,

No H5N1 allow, H5N1 non autoris?.

See it works when you have bilingual signs.

in English and French, I presume birds can ubderstand when both language are up in their fluways, oups, flyways :D
Click to expand...

Size matters in either language.

Canada knows the size of the insert from PEI (which was a pretty easy read - and Canada almost certainly has an electronic record).
 
Re: Low Path H5-N1 in Montana ducks

LOLLOLLOL

Snowy Owl said:
Canada is free of such events probably because of our bilingual signs,

No H5N1 allow, H5N1 non autoris?.

See it works when you have bilingual signs.

in English and French, I presume birds can ubderstand when both language are up in their fluways, oups, flyways :D
Click to expand...
 
Re: Low Path H5-N1 in Montana ducks

First line - :D

Second line - :confused: right over my head. ('sokay, I'm sure it was funny too)



[
QUOTE=niman]Size matters in either language.

Canada knows the size of the insert from PEI (which was a pretty easy read - and Canada almost certainly has an electronic record).[/QUOTE]
 
Re: Low Path H5-N1 in Montana ducks

Marcie said:
First line - :D

Second line - :confused: right over my head. ('sokay, I'm sure it was funny too)

[
QUOTE=niman]Size matters in either language.

Canada knows the size of the insert from PEI (which was a pretty easy read - and Canada almost certainly has an electronic record).
Click to expand...
[/quote]

The second line was an explanation of the first

http://www.recombinomics.com/News/06210603/H5_PCR_PEI.html

HPAI insert would be larger than LPAI inset.
 
Re: Low Path H5-N1 in Montana ducks

http://www.recombinomics.com/News/06210603/H5_PCR_PEI.html

<big><big>Commentary</big></big>

PCR Insert Contains Key H5 Sequence from Dead PEI Goose

Recombinomics Commentary
June 21, 2006

Using polymerase chain reaction or PCR testing, the scientists searched for tiny bits of genetic material from flu viruses. They spotted enough to declare that they had found an influenza A virus of the H5 subtype.

`They found a little piece that matched the H5, enough of a little piece to say it's H5. However, they can't tell if the virus is alive or not,'' Bosse says.

But there wasn't sufficient material to tell if it was highly pathogenic or a virus of low pathogenicity, or what the neuraminidase _ the N in a flu virus's name _ subtype was.

The above description of testing on Prince Edward Island raises questions about the insert generated in the PCR test as well as the sequence of the insert. The tiny bits of genetic information described above are primers. The primers bind to the genetic information of the virus, and then the polymerase reaction fills in the gap between the primers using the virus RNA as a template. Thus, the insert contains the sequence of the virus and it can be used to identify the viral sequence between the primers.

This insert was specific for H5, so it almost certainly included the HA cleavage site. The sequence of the HA cleavage site is diagnostic for high pathogenic avian influence (HPAI) or low pathogenic avian influenza (LPAI). Moreover, the GERRRKKR sequence at the cleavage site would indicate that the H5 was from the H5N1 Qinghai strain.

The Canadian Food Inspection Agency has maintained that the H5 in the dead goose was an incidental finding and the goose died of unknown causes. The key test of this hypothesis is the sequence of the HA cleavage site. If it has multiple basic amino acids, it is HPAI like the Qinghai strain of H5N1. If the multi-basic amino acids are missing, then it is LPAI bird flu, as suggested.

The isolation of H5 virus is unlikely because the samples at Winnipeg have degraded and are now PCR negative. In view of the state of the material in Winnipeg, the sequence of the insert created on Prince Edward Island should be released. This sequences would answer to key questions, is the H5 from HPAI and is it from the Qinghai strain of H5N1.
 
Re: Low Path H5-N1 in Montana ducks

<TABLE cellSpacing=0 cellPadding=0 width=612 border=0><TBODY><TR><TD class=BodyTextBlack colSpan=4 height=12>Release No. 0402.06</TD></TR><TR><TD class=BodyTextBlack align=right colSpan=4 height=12>Contact:</TD></TR><TR><TD class=BodyTextBlack align=right colSpan=4 height=12>Angela Harless, USDA (202) 841-0832</TD></TR><TR><TD class=BodyTextBlack align=right colSpan=4 height=12>DOI Press Office (202) 208-6416</TD></TR><TR><TD class=BodyTextBlackBold align=middle colSpan=4 height=10> </TD></TR><TR><TD class=BodyTextBlackBold vAlign=center align=middle colSpan=4 height=10>AVIAN INFLUENZA TESTS COMPLETE ON WILD NORTHERN PINTAIL DUCKS IN MONTANA</TD></TR><TR><TD class=BodyTextBlackBold align=middle colSpan=4 height=5></TD></TR><TR><TD class=BodyTextBlack colSpan=4 height=12>WASHINGTON, Oct. 7, 2006 - The U.S. Departments of Agriculture and Interior today announced final test results, which confirm that a low pathogenic H5 avian influenza virus was found in samples collected last month from wild Northern pintail ducks in Montana. This type of avian influenza has been detected several times in wild birds in North America and poses no risk to human health.
The USDA National Veterinary Services Laboratories (NVSL) confirmed the presence of low pathogenic H5N3 avian influenza through virus isolation in two of the 16 samples collected from wild pintails in Cascade County, Montana. Initial screening results announced on Sept. 21 indicated that H5 and N1 subtypes might be present in the collected samples, but further testing was necessary to confirm the H and N subtypes as well as pathogenicity.
The initial rapid screening tests are highly sensitive and can detect active and inactive viruses in samples. Varieties of this test can screen for the presence of all strains of avian influenza virus. Because these rapid screening tests are highly sensitive, it is not uncommon to have positive results for a specific subtype on the initial screen test and yet not be able to isolate a virus of that subtype. This was the case for the N1 subtype in this sample which tested as a weak positive in the initial screen test. During confirmatory testing, an N1 subtype was not isolated but instead an N3 was found. As previously announced, genetic testing ruled out the possibility that the samples carried the highly pathogenic strain of H5N1 avian influenza that is circulating overseas.
Low pathogenic strains of avian influenza occur naturally in wild birds and typically cause only minor sickness or no noticeable signs of disease in birds. In most cases it causes no signs of infection or only minor symptoms in birds. These strains are common in the U.S. and around the world. Low pathogenic avian influenza viruses are very different from the more severe highly pathogenic H5N1 circulating in parts of Asia, Europe and Africa. Highly pathogenic strains of avian influenza spread rapidly and are often fatal to chickens and turkeys.
The Departments of Agriculture and Interior are working collaboratively with States and academic institutions to sample wild birds throughout the United States for the presence of highly pathogenic avian influenza.
For more information about the collaborative avian influenza efforts go to http://www.usda.gov/birdflu , http://www.doi.gov/issues/avianflu or the U.S. Government's Web site for avian influenza and human pandemic preparedness at http://www.avianflu.gov
</TD></TR></TBODY></TABLE>
http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1RD?printable=true&contentidonly=true&contentid=2006/10/0402.xml
 
You must log in or register to reply here.
Back
Top