tetano
Editor, Senior Moderator
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110465. doi: 10.1016/j.msec.2019.110465. Epub 2019 Nov 19. [h=1]Low cost synthesis of reduced graphene oxide using biopolymer for influenza virus sensor.[/h]
Joshi SR[SUP]1[/SUP], Sharma A[SUP]2[/SUP], Kim GH[SUP]3[/SUP], Jang J[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A biocompatible, cost-effective, and scalable reduced graphene oxide (rGO) film was obtained from shellac using thermal treatment and its structural, chemical, and electrical properties were investigated. This thermally-decomposed rGO (TrGO) film exhibited good crystallinity, low sheet resistance, and high carbon content. TrGO flakes obtained from the film were dispersed and drop cast onto indium tin oxide/glass electrodes to fabricate label-free electrochemical immunosensors for the quantitative detection of the influenza virus H1N1 via electrochemical impedance spectroscopy. These sensors exhibited high stability and reproducibility, both possibly ascribable to the high adhesion of TrGO due to its phenolic-OH moiety; the limits of detection were 26 and 33 plaque-forming units, respectively, in phosphate-buffered saline and diluted saliva. These cost-effective TrGO-based sensors showed great potential as reliable and robust nanomaterial-based biosensors for widespread clinical applications.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] 2D carbon; Bio-waste; Electrochemical biosensor; Influenza virus; Thermal processing
PMID: 31924022 DOI: 10.1016/j.msec.2019.110465
Joshi SR[SUP]1[/SUP], Sharma A[SUP]2[/SUP], Kim GH[SUP]3[/SUP], Jang J[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A biocompatible, cost-effective, and scalable reduced graphene oxide (rGO) film was obtained from shellac using thermal treatment and its structural, chemical, and electrical properties were investigated. This thermally-decomposed rGO (TrGO) film exhibited good crystallinity, low sheet resistance, and high carbon content. TrGO flakes obtained from the film were dispersed and drop cast onto indium tin oxide/glass electrodes to fabricate label-free electrochemical immunosensors for the quantitative detection of the influenza virus H1N1 via electrochemical impedance spectroscopy. These sensors exhibited high stability and reproducibility, both possibly ascribable to the high adhesion of TrGO due to its phenolic-OH moiety; the limits of detection were 26 and 33 plaque-forming units, respectively, in phosphate-buffered saline and diluted saliva. These cost-effective TrGO-based sensors showed great potential as reliable and robust nanomaterial-based biosensors for widespread clinical applications.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] 2D carbon; Bio-waste; Electrochemical biosensor; Influenza virus; Thermal processing
PMID: 31924022 DOI: 10.1016/j.msec.2019.110465