tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2013 Jul 17. [Epub ahead of print]
Long-term protective immunity from an influenza virus-like particle vaccine administered with a microneedle patch.
Quan FS, Kim YC, Song JM, Hwang HS, Compans RW, Prausnitz MR, Kang SM.
Source
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Korea.
Abstract
Skin vaccination with influenza virus-like particles (VLPs) using microneedles has been shown to induce similar or better protection compared to intramuscular immunization. In this study, we examined the long-term protective efficacy of influenza (H1N1 A/PR/8/34) VLPs after skin vaccination using microneedle patches coated with the vaccine. Microneedle vaccination of mice in the skin induced 100% protection against lethal challenge infection with influenza A/PR/8/34 virus 14 months after a single vaccine dose. Influenza virus-specific total IgG response and hemagglutination inhibition (HAI) titers were maintained at high levels for over one year after microneedle vaccination. Microneedle vaccination also induced substantial levels of lung IgG and IgA antibody responses, and antibody-secreting plasma cells from spleen and bone marrow, as well as conferring effective control of lung viral loads, resulting in complete protection 14 months after vaccination. These strong and long-lasting immune responses were enabled in part by stabilization of the vaccine by formulation with trehalose during microneedle patch fabrication. Administration of the stabilized vaccine using microneedles was especially effective at enabling strong recall responses measured four days after lethal virus challenge, including increased HAI and antibody-secreting cells in the spleen and reduced viral titer and inflammatory response in the lung. The results in this study indicate that skin vaccination with VLP vaccine using a microneedle patch provides long-term protection against influenza in mice.
PMID:
23863506
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23863506
Long-term protective immunity from an influenza virus-like particle vaccine administered with a microneedle patch.
Quan FS, Kim YC, Song JM, Hwang HS, Compans RW, Prausnitz MR, Kang SM.
Source
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Korea.
Abstract
Skin vaccination with influenza virus-like particles (VLPs) using microneedles has been shown to induce similar or better protection compared to intramuscular immunization. In this study, we examined the long-term protective efficacy of influenza (H1N1 A/PR/8/34) VLPs after skin vaccination using microneedle patches coated with the vaccine. Microneedle vaccination of mice in the skin induced 100% protection against lethal challenge infection with influenza A/PR/8/34 virus 14 months after a single vaccine dose. Influenza virus-specific total IgG response and hemagglutination inhibition (HAI) titers were maintained at high levels for over one year after microneedle vaccination. Microneedle vaccination also induced substantial levels of lung IgG and IgA antibody responses, and antibody-secreting plasma cells from spleen and bone marrow, as well as conferring effective control of lung viral loads, resulting in complete protection 14 months after vaccination. These strong and long-lasting immune responses were enabled in part by stabilization of the vaccine by formulation with trehalose during microneedle patch fabrication. Administration of the stabilized vaccine using microneedles was especially effective at enabling strong recall responses measured four days after lethal virus challenge, including increased HAI and antibody-secreting cells in the spleen and reduced viral titer and inflammatory response in the lung. The results in this study indicate that skin vaccination with VLP vaccine using a microneedle patch provides long-term protection against influenza in mice.
PMID:
23863506
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23863506