tetano
Editor, Senior Moderator
Sci Rep. 2016 Apr 13;6:24402. doi: 10.1038/srep24402.
[h=1]Long-Lasting Cross-Protection Against Influenza A by Neuraminidase and M2e-based immunization strategies.[/h] Schotsaert M[SUP]1,[/SUP][SUP]2[/SUP], Ysenbaert T[SUP]1,[/SUP][SUP]2[/SUP], Smet A[SUP]1,[/SUP][SUP]2[/SUP], Schepens B[SUP]1,[/SUP][SUP]2[/SUP], Vanderschaeghe D[SUP]1,[/SUP][SUP]3[/SUP], Stegalkina S[SUP]4[/SUP], Vogel TU[SUP]4[/SUP], Callewaert N[SUP]1,[/SUP][SUP]3[/SUP], Fiers W[SUP]1,[/SUP][SUP]2[/SUP], Saelens X[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] There is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and H3N2 viruses of mice that had been immunized with hemagglutinin (HA), neuraminidase (NA) and the extracellular domain of matrix protein 2 (M2e) fused to a virus-like particle (VLP). Mice were challenged a first time with a sublethal dose of H1N1 2009 pandemic virus and, four weeks later, challenged again with an H3N2 virus. Mice that had been vaccinated with HA, NA, NA + M2e-VLP and HA + NA + M2e-VLP were protected against homologous H1N1 virus challenge. Challenged NA and NA + M2e-VLP vaccinated mice mounted CD8+ T cell responses that correlated with protection against secondary H3N2 challenge. HA-vaccinated mice were fully protected against challenge with homologous H1N1 2009 virus, failed to mount cross-reactive CD8+ T cells and succumbed to the second challenge with heterologous H3N2 virus. In summary, NA- and M2e-based immunity can protect against challenge with (homologous) virus without compromising the induction of robust cross-reactive CD8+ T cell responses upon exposure to virus.
PMID: 27072615 [PubMed - in process] Free full text
[h=1]Long-Lasting Cross-Protection Against Influenza A by Neuraminidase and M2e-based immunization strategies.[/h] Schotsaert M[SUP]1,[/SUP][SUP]2[/SUP], Ysenbaert T[SUP]1,[/SUP][SUP]2[/SUP], Smet A[SUP]1,[/SUP][SUP]2[/SUP], Schepens B[SUP]1,[/SUP][SUP]2[/SUP], Vanderschaeghe D[SUP]1,[/SUP][SUP]3[/SUP], Stegalkina S[SUP]4[/SUP], Vogel TU[SUP]4[/SUP], Callewaert N[SUP]1,[/SUP][SUP]3[/SUP], Fiers W[SUP]1,[/SUP][SUP]2[/SUP], Saelens X[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] There is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and H3N2 viruses of mice that had been immunized with hemagglutinin (HA), neuraminidase (NA) and the extracellular domain of matrix protein 2 (M2e) fused to a virus-like particle (VLP). Mice were challenged a first time with a sublethal dose of H1N1 2009 pandemic virus and, four weeks later, challenged again with an H3N2 virus. Mice that had been vaccinated with HA, NA, NA + M2e-VLP and HA + NA + M2e-VLP were protected against homologous H1N1 virus challenge. Challenged NA and NA + M2e-VLP vaccinated mice mounted CD8+ T cell responses that correlated with protection against secondary H3N2 challenge. HA-vaccinated mice were fully protected against challenge with homologous H1N1 2009 virus, failed to mount cross-reactive CD8+ T cells and succumbed to the second challenge with heterologous H3N2 virus. In summary, NA- and M2e-based immunity can protect against challenge with (homologous) virus without compromising the induction of robust cross-reactive CD8+ T cell responses upon exposure to virus.
PMID: 27072615 [PubMed - in process] Free full text