tetano
Editor, Senior Moderator
J Lipid Res. 2014 May 27. pii: jlr.M049148. [Epub ahead of print]
Lipidomics identifies a requirement for peroxisomal function during influenza virus replication.
Tanner LB1, Chng C1, Guan XL2, Lei Z3, Rozen SG3, Wenk MR4.
Author information
Abstract
Influenza virus acquires a host-derived lipid envelope during budding, yet a convergent view on the role of host lipid metabolism during infection is lacking. Using a mass spectrometry based lipidomics approach, we provide a systems-scale perspective on membrane lipid dynamics of infected human lung epithelial cells and purified influenza virions. We reveal enrichment of the minor peroxisome-derived ether-linked phosphatidylcholines relative to bulk ester-linked phosphatidylcholines in virions as a unique pathogenicity-dependent signature for influenza not found in other enveloped viruses. Strikingly, pharmacological and genetic interference with peroxisomal and ether lipid metabolism impaired influenza virus production. Further integration of our lipidomics results with published genomics and proteomics data corroborated altered peroxisomal lipid metabolism as a hallmark of influenza virus infection in vitro and in vivo. Influenza virus may therefore tailor peroxisomal and particularly ether lipid metabolism for efficient replication.
Copyright ? 2014, The American Society for Biochemistry and Molecular Biology.
KEYWORDS:
Ether Lipids; Influenza; Lipid Metabolism; Lipidomics; Peroxisomes; Phospholipids; Phospholipids/Metabolism; Sphingolipids; Systems Biology
PMID:
24868094
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24868094
Lipidomics identifies a requirement for peroxisomal function during influenza virus replication.
Tanner LB1, Chng C1, Guan XL2, Lei Z3, Rozen SG3, Wenk MR4.
Author information
Abstract
Influenza virus acquires a host-derived lipid envelope during budding, yet a convergent view on the role of host lipid metabolism during infection is lacking. Using a mass spectrometry based lipidomics approach, we provide a systems-scale perspective on membrane lipid dynamics of infected human lung epithelial cells and purified influenza virions. We reveal enrichment of the minor peroxisome-derived ether-linked phosphatidylcholines relative to bulk ester-linked phosphatidylcholines in virions as a unique pathogenicity-dependent signature for influenza not found in other enveloped viruses. Strikingly, pharmacological and genetic interference with peroxisomal and ether lipid metabolism impaired influenza virus production. Further integration of our lipidomics results with published genomics and proteomics data corroborated altered peroxisomal lipid metabolism as a hallmark of influenza virus infection in vitro and in vivo. Influenza virus may therefore tailor peroxisomal and particularly ether lipid metabolism for efficient replication.
Copyright ? 2014, The American Society for Biochemistry and Molecular Biology.
KEYWORDS:
Ether Lipids; Influenza; Lipid Metabolism; Lipidomics; Peroxisomes; Phospholipids; Phospholipids/Metabolism; Sphingolipids; Systems Biology
PMID:
24868094
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/24868094