tetano
Editor, Senior Moderator
Lancet Neurol
. 2020 Oct 5;S1474-4422(20)30308-2.
doi: 10.1016/S1474-4422(20)30308-2. Online ahead of print.
Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
Jakob Matschke[SUP] 1 [/SUP], Marc L?tgehetmann[SUP] 2 [/SUP], Christian Hagel[SUP] 1 [/SUP], Jan P Sperhake[SUP] 3 [/SUP], Ann Sophie Schr?der[SUP] 3 [/SUP], Carolin Edler[SUP] 3 [/SUP], Herbert Mushumba[SUP] 3 [/SUP], Antonia Fitzek[SUP] 3 [/SUP], Lena Allweiss[SUP] 4 [/SUP], Maura Dandri[SUP] 5 [/SUP], Matthias Dottermusch[SUP] 1 [/SUP], Axel Heinemann[SUP] 3 [/SUP], Susanne Pfefferle[SUP] 6 [/SUP], Marius Schwabenland[SUP] 7 [/SUP], Daniel Sumner Magruder[SUP] 8 [/SUP], Stefan Bonn[SUP] 9 [/SUP], Marco Prinz[SUP] 10 [/SUP], Christian Gerloff[SUP] 11 [/SUP], Klaus P?schel[SUP] 3 [/SUP], Susanne Krasemann[SUP] 1 [/SUP], Martin Aepfelbacher[SUP] 6 [/SUP], Markus Glatzel[SUP] 12 [/SUP]
Affiliations
Abstract
Background: Prominent clinical symptoms of COVID-19 include CNS manifestations. However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, gains access to the CNS and whether it causes neuropathological changes. We investigated the brain tissue of patients who died from COVID-19 for glial responses, inflammatory changes, and the presence of SARS-CoV-2 in the CNS.
Methods: In this post-mortem case series, we investigated the neuropathological features in the brains of patients who died between March 13 and April 24, 2020, in Hamburg, Germany. Inclusion criteria comprised a positive test for SARS-CoV-2 by quantitative RT-PCR (qRT-PCR) and availability of adequate samples. We did a neuropathological workup including histological staining and immunohistochemical staining for activated astrocytes, activated microglia, and cytotoxic T lymphocytes in the olfactory bulb, basal ganglia, brainstem, and cerebellum. Additionally, we investigated the presence and localisation of SARS-CoV-2 by qRT-PCR and by immunohistochemistry in selected patients and brain regions.
Findings: 43 patients were included in our study. Patients died in hospitals, nursing homes, or at home, and were aged between 51 years and 94 years (median 76 years [IQR 70-86]). We detected fresh territorial ischaemic lesions in six (14%) patients. 37 (86%) patients had astrogliosis in all assessed regions. Activation of microglia and infiltration by cytotoxic T lymphocytes was most pronounced in the brainstem and cerebellum, and meningeal cytotoxic T lymphocyte infiltration was seen in 34 (79%) patients. SARS-CoV-2 could be detected in the brains of 21 (53%) of 40 examined patients, with SARS-CoV-2 viral proteins found in cranial nerves originating from the lower brainstem and in isolated cells of the brainstem. The presence of SARS-CoV-2 in the CNS was not associated with the severity of neuropathological changes.
Interpretation: In general, neuropathological changes in patients with COVID-19 seem to be mild, with pronounced neuroinflammatory changes in the brainstem being the most common finding. There was no evidence for CNS damage directly caused by SARS-CoV-2. The generalisability of these findings needs to be validated in future studies as the number of cases and availability of clinical data were low and no age-matched and sex-matched controls were included.
Funding: German Research Foundation, Federal State of Hamburg, EU (eRARE), German Center for Infection Research (DZIF).
. 2020 Oct 5;S1474-4422(20)30308-2.
doi: 10.1016/S1474-4422(20)30308-2. Online ahead of print.
Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
Jakob Matschke[SUP] 1 [/SUP], Marc L?tgehetmann[SUP] 2 [/SUP], Christian Hagel[SUP] 1 [/SUP], Jan P Sperhake[SUP] 3 [/SUP], Ann Sophie Schr?der[SUP] 3 [/SUP], Carolin Edler[SUP] 3 [/SUP], Herbert Mushumba[SUP] 3 [/SUP], Antonia Fitzek[SUP] 3 [/SUP], Lena Allweiss[SUP] 4 [/SUP], Maura Dandri[SUP] 5 [/SUP], Matthias Dottermusch[SUP] 1 [/SUP], Axel Heinemann[SUP] 3 [/SUP], Susanne Pfefferle[SUP] 6 [/SUP], Marius Schwabenland[SUP] 7 [/SUP], Daniel Sumner Magruder[SUP] 8 [/SUP], Stefan Bonn[SUP] 9 [/SUP], Marco Prinz[SUP] 10 [/SUP], Christian Gerloff[SUP] 11 [/SUP], Klaus P?schel[SUP] 3 [/SUP], Susanne Krasemann[SUP] 1 [/SUP], Martin Aepfelbacher[SUP] 6 [/SUP], Markus Glatzel[SUP] 12 [/SUP]
Affiliations
- PMID: 33031735
- DOI: 10.1016/S1474-4422(20)30308-2
Abstract
Background: Prominent clinical symptoms of COVID-19 include CNS manifestations. However, it is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, gains access to the CNS and whether it causes neuropathological changes. We investigated the brain tissue of patients who died from COVID-19 for glial responses, inflammatory changes, and the presence of SARS-CoV-2 in the CNS.
Methods: In this post-mortem case series, we investigated the neuropathological features in the brains of patients who died between March 13 and April 24, 2020, in Hamburg, Germany. Inclusion criteria comprised a positive test for SARS-CoV-2 by quantitative RT-PCR (qRT-PCR) and availability of adequate samples. We did a neuropathological workup including histological staining and immunohistochemical staining for activated astrocytes, activated microglia, and cytotoxic T lymphocytes in the olfactory bulb, basal ganglia, brainstem, and cerebellum. Additionally, we investigated the presence and localisation of SARS-CoV-2 by qRT-PCR and by immunohistochemistry in selected patients and brain regions.
Findings: 43 patients were included in our study. Patients died in hospitals, nursing homes, or at home, and were aged between 51 years and 94 years (median 76 years [IQR 70-86]). We detected fresh territorial ischaemic lesions in six (14%) patients. 37 (86%) patients had astrogliosis in all assessed regions. Activation of microglia and infiltration by cytotoxic T lymphocytes was most pronounced in the brainstem and cerebellum, and meningeal cytotoxic T lymphocyte infiltration was seen in 34 (79%) patients. SARS-CoV-2 could be detected in the brains of 21 (53%) of 40 examined patients, with SARS-CoV-2 viral proteins found in cranial nerves originating from the lower brainstem and in isolated cells of the brainstem. The presence of SARS-CoV-2 in the CNS was not associated with the severity of neuropathological changes.
Interpretation: In general, neuropathological changes in patients with COVID-19 seem to be mild, with pronounced neuroinflammatory changes in the brainstem being the most common finding. There was no evidence for CNS damage directly caused by SARS-CoV-2. The generalisability of these findings needs to be validated in future studies as the number of cases and availability of clinical data were low and no age-matched and sex-matched controls were included.
Funding: German Research Foundation, Federal State of Hamburg, EU (eRARE), German Center for Infection Research (DZIF).