tetano
Editor, Senior Moderator
Biochem Biophys Res Commun. 2015 Oct 15. pii: S0006-291X(15)30767-1. doi: 10.1016/j.bbrc.2015.10.067. [Epub ahead of print]
[h=1]Lactoferrin acts as an adjuvant during influenza vaccination of neonatal mice.[/h] Sherman MP[SUP]1[/SUP], Pritzl CJ[SUP]2[/SUP], Xia C[SUP]3[/SUP], Miller MM[SUP]4[/SUP], Zaghouani H[SUP]5[/SUP], Hahm B[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Health policy precludes neonatal vaccination against influenza. Hence, morbidity and mortality are high under 6 months of age. Lactoferrin may activate diminished numbers of dysfunctional dendritic cells and reverse neonatal vaccine failures. Aluminum hydroxide/ALUM recruits neutrophils that secrete lactoferrin at deposition sites of antigen. We theorized lactoferrin + influenza antigen initiates an equivalent antibody response compared to ALUM. Three-day-old mice received subcutaneously 30 micrograms of H1N1 hemagglutinin + 200 μg of bovine lactoferrin versus hemagglutinin + ALUM. Controls received hemagglutinin, lactoferrin, or ALUM. After 21 days, sera measured anti-H1N1 (ELISA) and neutralizing antibody (plaque assays). ELISA detected equal antibody production with lactoferrin + hemagglutinin compared to hemagglutinin + ALUM; both sera also neutralized H1N1 virus at a 1:20 dilution (p<.01). Controls had no anti-H1N1 antibody. Neonates given lactoferrin had no anaphylaxis when challenged four weeks later. Lactoferrin is a safe and effective adjuvant for inducing antibody against influenza in neonates.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] adjuvant; hemagglutinin; immunization; influenza; lactoferrin; newborn
PMID: 26478433 [PubMed - as supplied by publisher]
[h=1]Lactoferrin acts as an adjuvant during influenza vaccination of neonatal mice.[/h] Sherman MP[SUP]1[/SUP], Pritzl CJ[SUP]2[/SUP], Xia C[SUP]3[/SUP], Miller MM[SUP]4[/SUP], Zaghouani H[SUP]5[/SUP], Hahm B[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Health policy precludes neonatal vaccination against influenza. Hence, morbidity and mortality are high under 6 months of age. Lactoferrin may activate diminished numbers of dysfunctional dendritic cells and reverse neonatal vaccine failures. Aluminum hydroxide/ALUM recruits neutrophils that secrete lactoferrin at deposition sites of antigen. We theorized lactoferrin + influenza antigen initiates an equivalent antibody response compared to ALUM. Three-day-old mice received subcutaneously 30 micrograms of H1N1 hemagglutinin + 200 μg of bovine lactoferrin versus hemagglutinin + ALUM. Controls received hemagglutinin, lactoferrin, or ALUM. After 21 days, sera measured anti-H1N1 (ELISA) and neutralizing antibody (plaque assays). ELISA detected equal antibody production with lactoferrin + hemagglutinin compared to hemagglutinin + ALUM; both sera also neutralized H1N1 virus at a 1:20 dilution (p<.01). Controls had no anti-H1N1 antibody. Neonates given lactoferrin had no anaphylaxis when challenged four weeks later. Lactoferrin is a safe and effective adjuvant for inducing antibody against influenza in neonates.
Copyright ? 2015. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] adjuvant; hemagglutinin; immunization; influenza; lactoferrin; newborn
PMID: 26478433 [PubMed - as supplied by publisher]