tetano
Editor, Senior Moderator
Epidemiol Mikrobiol Imunol. 2011 Feb;60(1):32-7.
[Laboratory diagnosis of pandemic influenza at the Department of Medical Microbiology of the Regional Authority of Public Health based in Bansk? Bystrica in the season 2009-2010].
[Article in Czech]
Kissov? R, Mad'arov? L, Klement C.
Source
Region?lny ?rad verejn?ho zdravotn?ctva so s?dlom v Banskej Bystrici, Odbor lek?rskej mikrobiol?gie. renata.kissova@vzbb.sk
Abstract
The Department of Medical Microbiology of the Regional Authority of Public Health (RAPH) in Bansk? Bystrica serves as a catchment laboratory of virology for the Central Slovakia Region, and in the influenza season 2009/10, it also served as such for the East Slovakia Region. Material and methods: Specimens (nasopharyngeal swabs and post-mortem specimens) from patients with suspected influenza were obtained from both sentinel and non-sentinel physicians. The specimens were analyzed by a rapid test, followed by real-time PCR (RT-PCR) for influenza A or B diagnosis. RT-PCR subtyping for pandemic influenza A/H1N1 was performed. Results: From May 2009 to June 2010, 2497 specimens were analyzed for the presence of influenza A and B viruses and in particular for the presence of pandemic influenza A/H1N1 virus. As many as 537 of 589 influenza A-positive specimens, i.e. 21.5% of all specimens analyzed and 91.2% of influenza A-positive specimens, were subtyped as pandemic influenza A/H1N1. Conclusion: In the influenza season 2009/10, the new pandemic influenza A/H1N1 clearly predominated in Central and Eastern Slovakia. PCR tests have played a key role in diagnosing patients with suspected pandemic influenza in the laboratory participating in the surveillance of influenza and influenza-like illness in the Slovak Republic.
PMID:
21542233
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21542233
[Laboratory diagnosis of pandemic influenza at the Department of Medical Microbiology of the Regional Authority of Public Health based in Bansk? Bystrica in the season 2009-2010].
[Article in Czech]
Kissov? R, Mad'arov? L, Klement C.
Source
Region?lny ?rad verejn?ho zdravotn?ctva so s?dlom v Banskej Bystrici, Odbor lek?rskej mikrobiol?gie. renata.kissova@vzbb.sk
Abstract
The Department of Medical Microbiology of the Regional Authority of Public Health (RAPH) in Bansk? Bystrica serves as a catchment laboratory of virology for the Central Slovakia Region, and in the influenza season 2009/10, it also served as such for the East Slovakia Region. Material and methods: Specimens (nasopharyngeal swabs and post-mortem specimens) from patients with suspected influenza were obtained from both sentinel and non-sentinel physicians. The specimens were analyzed by a rapid test, followed by real-time PCR (RT-PCR) for influenza A or B diagnosis. RT-PCR subtyping for pandemic influenza A/H1N1 was performed. Results: From May 2009 to June 2010, 2497 specimens were analyzed for the presence of influenza A and B viruses and in particular for the presence of pandemic influenza A/H1N1 virus. As many as 537 of 589 influenza A-positive specimens, i.e. 21.5% of all specimens analyzed and 91.2% of influenza A-positive specimens, were subtyped as pandemic influenza A/H1N1. Conclusion: In the influenza season 2009/10, the new pandemic influenza A/H1N1 clearly predominated in Central and Eastern Slovakia. PCR tests have played a key role in diagnosing patients with suspected pandemic influenza in the laboratory participating in the surveillance of influenza and influenza-like illness in the Slovak Republic.
PMID:
21542233
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/21542233