tetano
Editor, Senior Moderator
Open Virol J. 2012;6:151-9. doi: 10.2174/1874357901206010151. Epub 2012 Nov 30.
Laboratory detection of respiratory viruses by automated techniques.
P?rez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-G?mez S, Navarro-Mar? M.
Source
Laboratorio de Referencia de Salud P?blica para Enfermedades con Sospecha de Etiolog?a V?rica en Andaluc?a (Consejer?a de Salud), Servicio de Microbiolog?a, Hospital Universitario Virgen de las Nieves, Avda, Fuerzas Armadas, 2, 18014 Granada, Spain.
Abstract
Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.
PMID:
23248735
[PubMed]
PMCID:
PMC3522051
http://www.ncbi.nlm.nih.gov/pubmed/23248735
Laboratory detection of respiratory viruses by automated techniques.
P?rez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-G?mez S, Navarro-Mar? M.
Source
Laboratorio de Referencia de Salud P?blica para Enfermedades con Sospecha de Etiolog?a V?rica en Andaluc?a (Consejer?a de Salud), Servicio de Microbiolog?a, Hospital Universitario Virgen de las Nieves, Avda, Fuerzas Armadas, 2, 18014 Granada, Spain.
Abstract
Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.
PMID:
23248735
[PubMed]
PMCID:
PMC3522051
http://www.ncbi.nlm.nih.gov/pubmed/23248735