tetano
Editor, Senior Moderator
Polymers (Basel). 2017 Mar 30;9(4). pii: E127. doi: 10.3390/polym9040127.
[h=1]Label-Free Colorimetric Detection of Influenza Antigen Based on an Antibody-Polydiacetylene Conjugate and Its Coated Polyvinylidene Difluoride Membrane.[/h] Jeong JP[SUP]1[/SUP], Cho E[SUP]2[/SUP], Yun D[SUP]3[/SUP], Kim T[SUP]4[/SUP], Lee IS[SUP]5[/SUP], Jung S[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study presents an antibody-conjugated polydiacetylene (PDA) and its coated polyvinylidene difluoride (PVDF) membrane. The M149 antibody was hybridized to nano-vesicles consisting of pentacosa-10,12-diynoic acid (PCDA) and dimyristoylphosphatidylcholine (DMPC). After photo-polymerization at 254 nm, the effects on the PDA by antigenic injection were investigated with UV-vis spectroscopy, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. Because PDA, an alternating ene-yne molecule, induces a blue-to-red color transition and an interesting fluorescent response by the distortion of its backbone, the biomolecular recognition of an antibody⁻antigen can be converted into an optical and fluorescent signal. Thus, an influenza antigen was successfully detected with the proposed label-free method. Furthermore, the vesicular system was improved by coating it onto a membrane type sensing platform for its stability and portability. The proposed antibody-PDA composite PVDF membrane has potential for rapid, easy and selective visualization of the influenza virus.
[h=4]KEYWORDS:[/h] antibody; influenza virus; label-free detection; polydiacetylene; polyvinylidene difluoride
PMID: 30970806 DOI: 10.3390/polym9040127
[h=1]Label-Free Colorimetric Detection of Influenza Antigen Based on an Antibody-Polydiacetylene Conjugate and Its Coated Polyvinylidene Difluoride Membrane.[/h] Jeong JP[SUP]1[/SUP], Cho E[SUP]2[/SUP], Yun D[SUP]3[/SUP], Kim T[SUP]4[/SUP], Lee IS[SUP]5[/SUP], Jung S[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This study presents an antibody-conjugated polydiacetylene (PDA) and its coated polyvinylidene difluoride (PVDF) membrane. The M149 antibody was hybridized to nano-vesicles consisting of pentacosa-10,12-diynoic acid (PCDA) and dimyristoylphosphatidylcholine (DMPC). After photo-polymerization at 254 nm, the effects on the PDA by antigenic injection were investigated with UV-vis spectroscopy, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. Because PDA, an alternating ene-yne molecule, induces a blue-to-red color transition and an interesting fluorescent response by the distortion of its backbone, the biomolecular recognition of an antibody⁻antigen can be converted into an optical and fluorescent signal. Thus, an influenza antigen was successfully detected with the proposed label-free method. Furthermore, the vesicular system was improved by coating it onto a membrane type sensing platform for its stability and portability. The proposed antibody-PDA composite PVDF membrane has potential for rapid, easy and selective visualization of the influenza virus.
[h=4]KEYWORDS:[/h] antibody; influenza virus; label-free detection; polydiacetylene; polyvinylidene difluoride
PMID: 30970806 DOI: 10.3390/polym9040127