tetano
Editor, Senior Moderator
Korean J Intern Med
. 2021 Sep 27.
doi: 10.3904/kjim.2021.093. Online ahead of print.
Eosinophil-mediated lung inflammation associated with elevated natural killer T cell response in COVID-19 patients
Dong-Min Kim[SUP] 1 [/SUP], Jun-Won Seo[SUP] 1 [/SUP], Yuri Kim[SUP] 2 [/SUP], Uni Park[SUP] 2 [/SUP], Na-Young Ha[SUP] 2 [/SUP], Hyoree Park[SUP] 2 [/SUP], Na Ra Yun[SUP] 1 [/SUP], Da Young Kim[SUP] 1 [/SUP], Sung Ho Yoon[SUP] 1 [/SUP], Yong Sub Na[SUP] 1 [/SUP], Do Sik Moon[SUP] 1 [/SUP], Sung-Chul Lim[SUP] 3 [/SUP], Choon-Mee Kim[SUP] 4 [/SUP], Yeon-Sook Kim[SUP] 5 [/SUP], Nam-Hyuk Cho[SUP] 2 [/SUP]
Affiliations
Abstract
Background/aims: Coronavirus disease 2019 (COVID-19) is associated with acute respiratory syndrome. The mechanisms underlying the different degrees of pneumonia severity in patients with COVID-19 remain elusive. This study provides evidence that COVID-19 is associated with eosinophil-mediated inflammation.
Methods: We performed a retrospective case series of three patients with laboratory and radiologically confirmed COVID-19 pneumonia admitted to Chosun University Hospital. Demographic and clinical data on inflammatory cell lung infiltration and cytokine levels in patients with COVID-19 were collected.
Results: Cytological analysis of sputum, tracheal aspirates, and bronchoalveolar lavage fluid (BALF) samples from all three patients revealed massive infiltration of polymorphonuclear cells (PMNs), such as eosinophils and neutrophils. All sputum and BALF specimens contained high levels of eosinophil cationic proteins. The infiltration of PMNs into the lungs, together with elevated levels of natural killer T (NKT) cells in BALF and peripheral blood samples from patients with severe pneumonia in the acute phase was confirmed by flow cytometry.
Conclusions: These results suggest that the lungs of COVID-19 patients can exhibit eosinophil-mediated inflammation, together with an elevated NKT cell response, which is associated with COVID-19 pneumonia.
Keywords: Bronchoalveolar lavage; COVID-19; Eosinophil; Natural killer T-cells; SARS-CoV-2.
. 2021 Sep 27.
doi: 10.3904/kjim.2021.093. Online ahead of print.
Eosinophil-mediated lung inflammation associated with elevated natural killer T cell response in COVID-19 patients
Dong-Min Kim[SUP] 1 [/SUP], Jun-Won Seo[SUP] 1 [/SUP], Yuri Kim[SUP] 2 [/SUP], Uni Park[SUP] 2 [/SUP], Na-Young Ha[SUP] 2 [/SUP], Hyoree Park[SUP] 2 [/SUP], Na Ra Yun[SUP] 1 [/SUP], Da Young Kim[SUP] 1 [/SUP], Sung Ho Yoon[SUP] 1 [/SUP], Yong Sub Na[SUP] 1 [/SUP], Do Sik Moon[SUP] 1 [/SUP], Sung-Chul Lim[SUP] 3 [/SUP], Choon-Mee Kim[SUP] 4 [/SUP], Yeon-Sook Kim[SUP] 5 [/SUP], Nam-Hyuk Cho[SUP] 2 [/SUP]
Affiliations
- PMID: 34565131
- DOI: 10.3904/kjim.2021.093
Abstract
Background/aims: Coronavirus disease 2019 (COVID-19) is associated with acute respiratory syndrome. The mechanisms underlying the different degrees of pneumonia severity in patients with COVID-19 remain elusive. This study provides evidence that COVID-19 is associated with eosinophil-mediated inflammation.
Methods: We performed a retrospective case series of three patients with laboratory and radiologically confirmed COVID-19 pneumonia admitted to Chosun University Hospital. Demographic and clinical data on inflammatory cell lung infiltration and cytokine levels in patients with COVID-19 were collected.
Results: Cytological analysis of sputum, tracheal aspirates, and bronchoalveolar lavage fluid (BALF) samples from all three patients revealed massive infiltration of polymorphonuclear cells (PMNs), such as eosinophils and neutrophils. All sputum and BALF specimens contained high levels of eosinophil cationic proteins. The infiltration of PMNs into the lungs, together with elevated levels of natural killer T (NKT) cells in BALF and peripheral blood samples from patients with severe pneumonia in the acute phase was confirmed by flow cytometry.
Conclusions: These results suggest that the lungs of COVID-19 patients can exhibit eosinophil-mediated inflammation, together with an elevated NKT cell response, which is associated with COVID-19 pneumonia.
Keywords: Bronchoalveolar lavage; COVID-19; Eosinophil; Natural killer T-cells; SARS-CoV-2.