tetano
Editor, Senior Moderator
Int J Antimicrob Agents. 2015 Apr 20. pii: S0924-8579(15)00145-4. doi: 10.1016/j.ijantimicag.2015.03.004. [Epub ahead of print]
[h=1]Knockdown of different influenza A virus subtypes in cell culture by a single antisense oligodeoxyribonucleotide.[/h] Levina AS[SUP]1[/SUP], Repkova MN[SUP]1[/SUP], Mazurkova NA[SUP]2[/SUP], Makarevich EV[SUP]2[/SUP], Ismagilov ZR[SUP]3[/SUP], Zarytova VF[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a heavy socially significant viral infection that affects humans, birds, and wild and domestic animals. The threat of existing and new highly pathogenic subtypes of influenza A virus (IAV) makes it necessary to develop an effective drug that may affect different IAV strains. For this purpose, oligodeoxynucleotides (DNA fragments) attached to titanium dioxide (TiO[SUB]2[/SUB]) nanoparticles through a polylysine linker, forming TiO[SUB]2[/SUB]?PL-DNA nanocomposites, that penetrated into cells without transfection agents were used. For the first time, efficient (≥99.9%) suppression of the reproduction of different subtypes of IAV, including highly pathogenic H5N1 and H1N1, was achieved. These results were obtained using the TiO[SUB]2[/SUB]?PL-DNA nanocomposite bearing a single antisense oligodeoxynucleotide (0.1μM) targeted to the conserved 3'-noncoding region of RNA segment 5, which is common to all tested strains. Very efficient suppression of the reproduction of different subtypes of IAV was probably achieved due to the use of the proposed delivery system for oligonucleotides in the form of the TiO[SUB]2[/SUB]?PL-DNA nanocomposites. These results indicate the possibility of creating an efficient drug to affect existing and newly emerging pathogenic IAV strains.
Copyright ? 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
[h=4]KEYWORDS:[/h] DNA fragments; Influenza A virus; Nanocomposite; TiO(2) nanoparticles
PMID: 25963340 [PubMed - as supplied by publisher]
[h=1]Knockdown of different influenza A virus subtypes in cell culture by a single antisense oligodeoxyribonucleotide.[/h] Levina AS[SUP]1[/SUP], Repkova MN[SUP]1[/SUP], Mazurkova NA[SUP]2[/SUP], Makarevich EV[SUP]2[/SUP], Ismagilov ZR[SUP]3[/SUP], Zarytova VF[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a heavy socially significant viral infection that affects humans, birds, and wild and domestic animals. The threat of existing and new highly pathogenic subtypes of influenza A virus (IAV) makes it necessary to develop an effective drug that may affect different IAV strains. For this purpose, oligodeoxynucleotides (DNA fragments) attached to titanium dioxide (TiO[SUB]2[/SUB]) nanoparticles through a polylysine linker, forming TiO[SUB]2[/SUB]?PL-DNA nanocomposites, that penetrated into cells without transfection agents were used. For the first time, efficient (≥99.9%) suppression of the reproduction of different subtypes of IAV, including highly pathogenic H5N1 and H1N1, was achieved. These results were obtained using the TiO[SUB]2[/SUB]?PL-DNA nanocomposite bearing a single antisense oligodeoxynucleotide (0.1μM) targeted to the conserved 3'-noncoding region of RNA segment 5, which is common to all tested strains. Very efficient suppression of the reproduction of different subtypes of IAV was probably achieved due to the use of the proposed delivery system for oligonucleotides in the form of the TiO[SUB]2[/SUB]?PL-DNA nanocomposites. These results indicate the possibility of creating an efficient drug to affect existing and newly emerging pathogenic IAV strains.
Copyright ? 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
[h=4]KEYWORDS:[/h] DNA fragments; Influenza A virus; Nanocomposite; TiO(2) nanoparticles
PMID: 25963340 [PubMed - as supplied by publisher]