JVI: The NS1 protein of influenza A virus blocks RIG-I mediated activation of the noncanonical NF-κB pathway and p52/RelB dependent gene expression

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Published ahead of print 11 July 2012, doi: 10.1128/​JVI.00323-12



The NS1 protein of influenza A virus blocks RIG-I mediated activation of the noncanonical NF-κB pathway and p52/RelB dependent gene expression in lung epithelial cells

Andrea R?ckle1,
Emanuel Haasbach2,
Ilkka Julkunen3,
Oliver Planz2,
Christina Ehrhardt1 and
Stephan Ludwig1,*

1Institute of Molecular Virology (IMV), Centre for Molecular Biology of Inflammation (ZMBE), Westf?lische-Wilhelms-University, Von-Esmarch-Str. 56, 48149 Muenster, Germany
2Interfaculty Institute for Cell Biology, Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tuebingen, Germany
3Virology Unit, National Institute for Health and Welfare (THL), Mannerheimintie 166, 00300 Helsinki, Finland

ABSTRACT

Influenza A virus (IAV) infection of epithelial cells activates NF-κB transcription factors via the canonical NF-κB signaling pathway which modulates both, the antiviral immune response and viral replication. Since almost nothing is known so far about a function of noncanonical NF-κB signaling after IAV infection, we tested infected cells for activation of p52 and RelB. We show that the viral NS1 strongly inhibits RIG-I-mediated noncanonical NF-κB activation and expression of the noncanonical target gene CCL19.


http://jvi.asm.org/content/early/2012/07/05/JVI.00323-12.abstract