tetano
Editor, Senior Moderator
Published ahead of print 26 September 2012, doi: 10.1128/JVI.01820-12
Pseudotyped Influenza A Virus as a Vaccine for the induction of Heterotypic Immunity
Timothy J. Powell1,
Jonathan D. Silk2,
Jane Sharps3,
Ervin Fodor3 and
Alain R. M. Townsend1,*
1Molecular Immunology Group
2MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS
3Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
ABSTRACT
There is a need for vaccines that can protect broadly across all Influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase, but cannot replicate. We show that when given by inhalation to mice S-FLU is non-pathogenic, but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.
http://jvi.asm.org/content/early/2012/09/20/JVI.01820-12.abstract
Pseudotyped Influenza A Virus as a Vaccine for the induction of Heterotypic Immunity
Timothy J. Powell1,
Jonathan D. Silk2,
Jane Sharps3,
Ervin Fodor3 and
Alain R. M. Townsend1,*
1Molecular Immunology Group
2MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS
3Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
ABSTRACT
There is a need for vaccines that can protect broadly across all Influenza A strains. We have produced a pseudotyped influenza virus based on suppression of the A/PR/8/34 hemagglutinin signal sequence (S-FLU) that can infect cells and express the viral core proteins and neuraminidase, but cannot replicate. We show that when given by inhalation to mice S-FLU is non-pathogenic, but generates a vigorous T cell response in the lung associated with markedly reduced viral titers and weight loss after challenge with H1 and H3 influenza viruses. These properties of S-FLU suggest that it may have potential as a broadly protective A virus vaccine, particularly in the setting of a threatened pandemic before matched subunit vaccines become available.
http://jvi.asm.org/content/early/2012/09/20/JVI.01820-12.abstract