tetano
Editor, Senior Moderator
Published ahead of print 9 April 2014, doi: 10.1128/JVI.00420-14 JVI.00420-14
Conserved Neutralizing Epitope at Globular Head of Hemagglutinin in H3N2 Influenza Viruses
Yoshitaka Ibaa,
Yoshifumi Fujiib,
Nobuko Ohshimaa,
Tomomi Sumidab,
Ritsuko Kubota-Koketsuc,
Mariko Ikedab,
Motoaki Wakiyamab,
Mikako Shirouzub,
Jun Okadad,
Yoshinobu Okunoe,
Yoshikazu Kurosawaa⇑ and
Shigeyuki Yokoyamab
aInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan
bRIKEN Systems and Structural Biology Center, Suehiro, Tsurumi, Yokohama 230-0045, Japan
cDepartment of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
dSales & Marketing Division, Medical & Biological Laboratories (MBL) Co., Ltd., Nagoya, Aichi 460-0002, Japan
eKanonji Institute, The Research Foundation for Microbial Diseases, Osaka University, Kanonji, Kagawa 768-0061, Japan
ABSTRACT
Neutralizing antibodies that target the hemagglutinin of influenza virus either inhibit binding of hemagglutinin to cellular receptors or prevent the low-pH-induced conformational change in hemagglutinin required for membrane fusion. In general, the former type of antibody binds to the globular head formed by HA1 and has narrow strain specificity, while the latter type binds to the stem mainly formed by HA2 and has broad strain specificity. In the present study, we analyzed the epitope and function of a broadly neutralizing human antibody against H3N2 viruses, F005-126. The crystal structure of F005-126 Fab in complex with hemagglutinin revealed that the antibody binds to the globular head, spans a cleft formed by two hemagglutinin monomers in a hemagglutinin trimer, and cross-links them. It recognizes two peptide portions (sites L and R) and a glycan linked to asparagine at residue 285 using three complementarity-determining regions and framework 3 in the heavy chain. Binding of the antibody to sites L (residues 171-173, 239 and 240) and R (residues 91, 92, 270-273, 284 and 285) is mediated mainly by van der Waals contacts with the main chains of the peptides in these sites and secondarily by hydrogen bonds with a few side chains of conserved sequences in HA1. Furthermore, the glycan recognized by F005-126 is conserved among H3N2 viruses. F005-126 has the ability to prevent low-pH-induced conformational changes in hemagglutinin. The newly identified conserved epitope including the glycan should be immunogenic in humans, and may induce production of broadly neutralizing antibodies against H3 viruses.
IMPORTANCE Antibodies play an important role in protection against influenza virus, and hemagglutinin is the major target for virus neutralizing Abs. It has long been believed that all effective neutralizing antibodies bind to the surrounding regions of the sialic acid-binding pocket and inhibit the binding of hemagglutinin to the cellular receptor. As mutations are readily introduced into such epitopes, this type of antibody shows narrow strain specificity. Recently, however, broadly neutralizing antibodies have been isolated. Most of these bind either to conserved sites in the stem region or to the sialic acid-binding pocket itself. In the present study, we identified a new neutralizing epitope in the head region recognized by a broadly neutralizing human antibody against H3N2. This epitope may be useful for design of vaccines.
open access
http://jvi.asm.org/content/early/2014/04/03/JVI.00420-14.abstract
Conserved Neutralizing Epitope at Globular Head of Hemagglutinin in H3N2 Influenza Viruses
Yoshitaka Ibaa,
Yoshifumi Fujiib,
Nobuko Ohshimaa,
Tomomi Sumidab,
Ritsuko Kubota-Koketsuc,
Mariko Ikedab,
Motoaki Wakiyamab,
Mikako Shirouzub,
Jun Okadad,
Yoshinobu Okunoe,
Yoshikazu Kurosawaa⇑ and
Shigeyuki Yokoyamab
aInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan
bRIKEN Systems and Structural Biology Center, Suehiro, Tsurumi, Yokohama 230-0045, Japan
cDepartment of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
dSales & Marketing Division, Medical & Biological Laboratories (MBL) Co., Ltd., Nagoya, Aichi 460-0002, Japan
eKanonji Institute, The Research Foundation for Microbial Diseases, Osaka University, Kanonji, Kagawa 768-0061, Japan
ABSTRACT
Neutralizing antibodies that target the hemagglutinin of influenza virus either inhibit binding of hemagglutinin to cellular receptors or prevent the low-pH-induced conformational change in hemagglutinin required for membrane fusion. In general, the former type of antibody binds to the globular head formed by HA1 and has narrow strain specificity, while the latter type binds to the stem mainly formed by HA2 and has broad strain specificity. In the present study, we analyzed the epitope and function of a broadly neutralizing human antibody against H3N2 viruses, F005-126. The crystal structure of F005-126 Fab in complex with hemagglutinin revealed that the antibody binds to the globular head, spans a cleft formed by two hemagglutinin monomers in a hemagglutinin trimer, and cross-links them. It recognizes two peptide portions (sites L and R) and a glycan linked to asparagine at residue 285 using three complementarity-determining regions and framework 3 in the heavy chain. Binding of the antibody to sites L (residues 171-173, 239 and 240) and R (residues 91, 92, 270-273, 284 and 285) is mediated mainly by van der Waals contacts with the main chains of the peptides in these sites and secondarily by hydrogen bonds with a few side chains of conserved sequences in HA1. Furthermore, the glycan recognized by F005-126 is conserved among H3N2 viruses. F005-126 has the ability to prevent low-pH-induced conformational changes in hemagglutinin. The newly identified conserved epitope including the glycan should be immunogenic in humans, and may induce production of broadly neutralizing antibodies against H3 viruses.
IMPORTANCE Antibodies play an important role in protection against influenza virus, and hemagglutinin is the major target for virus neutralizing Abs. It has long been believed that all effective neutralizing antibodies bind to the surrounding regions of the sialic acid-binding pocket and inhibit the binding of hemagglutinin to the cellular receptor. As mutations are readily introduced into such epitopes, this type of antibody shows narrow strain specificity. Recently, however, broadly neutralizing antibodies have been isolated. Most of these bind either to conserved sites in the stem region or to the sialic acid-binding pocket itself. In the present study, we identified a new neutralizing epitope in the head region recognized by a broadly neutralizing human antibody against H3N2. This epitope may be useful for design of vaccines.
open access
http://jvi.asm.org/content/early/2014/04/03/JVI.00420-14.abstract