tetano
Editor, Senior Moderator
Nanomedicine. 2013 Nov 22. pii: S1549-9634(13)00596-0. doi: 10.1016/j.nano.2013.11.007. [Epub ahead of print]
Just-in-time vaccines: Biomineralized calcium phosphate core-immunogen shell nanoparticles induce long-lasting CD8+ T cell responses in mice.
Zhou W, Moguche A, Chiu D, Murali-Krishna K, Baneyx F.
Source
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Abstract
Distributed and on-demand vaccine production could be game-changing for infectious disease treatment in the developing world by providing new therapeutic opportunities and breaking the refrigeration "cold chain". Here, we show that a fusion protein between a calcium phosphate binding domain and the model antigen ovalbumin can mineralize a biocompatible adjuvant in a single step. The resulting 50nm calcium phosphate core-immunogen shell particles are comparable to soluble protein in inducing ovalbumin-specific antibody response and class switch recombination in mice. However, single dose vaccination with nanoparticles leads to higher expansion of ovalbumin-specific CD8+ T cells upon challenge with an influenza virus bearing the ovalbumin-derived SIINFEKL peptide, and these cells produce high levels of IFN-γ. Furthermore, mice exhibit a robust antigen-specific CD8+ T cell recall response when challenged with virus 8months post-immunization. These results underscore the promise of immunogen-controlled adjuvant mineralization for just-in-time manufacturing of effective T cell vaccines.
Copyright ? 2013. Published by Elsevier Inc.
KEYWORDS:
Biomineralization, Nanocarrier, Nanovaccine, Solid binding peptide
PMID:
24275478
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24275478
Just-in-time vaccines: Biomineralized calcium phosphate core-immunogen shell nanoparticles induce long-lasting CD8+ T cell responses in mice.
Zhou W, Moguche A, Chiu D, Murali-Krishna K, Baneyx F.
Source
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Abstract
Distributed and on-demand vaccine production could be game-changing for infectious disease treatment in the developing world by providing new therapeutic opportunities and breaking the refrigeration "cold chain". Here, we show that a fusion protein between a calcium phosphate binding domain and the model antigen ovalbumin can mineralize a biocompatible adjuvant in a single step. The resulting 50nm calcium phosphate core-immunogen shell particles are comparable to soluble protein in inducing ovalbumin-specific antibody response and class switch recombination in mice. However, single dose vaccination with nanoparticles leads to higher expansion of ovalbumin-specific CD8+ T cells upon challenge with an influenza virus bearing the ovalbumin-derived SIINFEKL peptide, and these cells produce high levels of IFN-γ. Furthermore, mice exhibit a robust antigen-specific CD8+ T cell recall response when challenged with virus 8months post-immunization. These results underscore the promise of immunogen-controlled adjuvant mineralization for just-in-time manufacturing of effective T cell vaccines.
Copyright ? 2013. Published by Elsevier Inc.
KEYWORDS:
Biomineralization, Nanocarrier, Nanovaccine, Solid binding peptide
PMID:
24275478
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24275478