Giuseppe
Emeritus
The Lack of an Inherent Membrane Targeting Signal is Responsible for the Failure of the Matrix (M1) Protein of Influenza A Virus to Bud into Virus-Like-Particles. (J Virol., abstract, edited)
9. J Virol. 2010 Feb 24. [Epub ahead of print]
The Lack of an Inherent Membrane Targeting Signal is Responsible for the Failure of the Matrix (M1) Protein of Influenza A Virus to Bud into Virus-Like-Particles.
Wang D, Harmon A, Jin J, Francis DH, Christopher-Hennings J, Nelson E, Montelaro RC, Li F. - Department of Biology and Microbiology, Department of Veterinary Science, Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007; Department of Microbiology and Molecular Genetics, Center for Vaccine Research, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
The matrix protein (M1) of influenza A virus is generally viewed as a key orchestrator in the release of influenza virions from the plasma membrane during infection. In contrast to this model, recent studies have indicated that influenza virus requires expression of the envelope proteins for budding of intracellular M1 into virus particles. Here we explored the mechanisms that control M1 budding. Similar to previous studies, we found that M1 by itself fails to form virus-like-particles (VLPs). We further demonstrated that M1, in the absence of other viral proteins, was preferentially targeted to the nucleus/perinuclear region rather than to the plasma membrane where influenza virions bud. Remarkably, we showed that a 10-residue membrane targeting peptide from either the Fyn or Lck oncoproteins appended to M1 at the N-terminus redirected M1 to the plasma membrane and allowed M1 particle budding without additional viral envelope proteins. To further identify a functional link between plasma membrane targeting and VLP formation, we took advantage of the fact that M1 can interact with M2, unless the cytoplasmic tail is absent. Notably, native M2 not mutant M2 effectively targeted M1 to the plasma membrane and produced extracellular M1 VLPs. Our results suggest that influenza M1 may not possess an inherent membrane-targeting signal. Thus, the lack of efficient plasma membrane targeting is responsible for the failure of M1 in budding. This study highlights the fact that interactions of M1 with viral envelope proteins are essential to direct M1 to the plasma membrane for influenza particle release.
PMID: 20181696 [PubMed - as supplied by publisher]
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9. J Virol. 2010 Feb 24. [Epub ahead of print]
The Lack of an Inherent Membrane Targeting Signal is Responsible for the Failure of the Matrix (M1) Protein of Influenza A Virus to Bud into Virus-Like-Particles.
Wang D, Harmon A, Jin J, Francis DH, Christopher-Hennings J, Nelson E, Montelaro RC, Li F. - Department of Biology and Microbiology, Department of Veterinary Science, Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007; Department of Microbiology and Molecular Genetics, Center for Vaccine Research, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
The matrix protein (M1) of influenza A virus is generally viewed as a key orchestrator in the release of influenza virions from the plasma membrane during infection. In contrast to this model, recent studies have indicated that influenza virus requires expression of the envelope proteins for budding of intracellular M1 into virus particles. Here we explored the mechanisms that control M1 budding. Similar to previous studies, we found that M1 by itself fails to form virus-like-particles (VLPs). We further demonstrated that M1, in the absence of other viral proteins, was preferentially targeted to the nucleus/perinuclear region rather than to the plasma membrane where influenza virions bud. Remarkably, we showed that a 10-residue membrane targeting peptide from either the Fyn or Lck oncoproteins appended to M1 at the N-terminus redirected M1 to the plasma membrane and allowed M1 particle budding without additional viral envelope proteins. To further identify a functional link between plasma membrane targeting and VLP formation, we took advantage of the fact that M1 can interact with M2, unless the cytoplasmic tail is absent. Notably, native M2 not mutant M2 effectively targeted M1 to the plasma membrane and produced extracellular M1 VLPs. Our results suggest that influenza M1 may not possess an inherent membrane-targeting signal. Thus, the lack of efficient plasma membrane targeting is responsible for the failure of M1 in budding. This study highlights the fact that interactions of M1 with viral envelope proteins are essential to direct M1 to the plasma membrane for influenza particle release.
PMID: 20181696 [PubMed - as supplied by publisher]
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