tetano
Editor, Senior Moderator
J Virol
. 2020 Sep 23;JVI.01091-20.
doi: 10.1128/JVI.01091-20. Online ahead of print.
Primary swine respiratory epithelial cell lines for the efficient isolation and propagation of influenza A viruses
Victoria Meliopoulos[SUP] 1 [/SUP], Sean Cherry[SUP] 2 [/SUP], Nicholas Wohlgemuth[SUP] 2 [/SUP], Rebekah Honce[SUP] 2 3 [/SUP], Karen Barnard[SUP] 4 [/SUP], Phillip Gauger[SUP] 5 [/SUP], Todd Davis[SUP] 5 [/SUP], Peter Shult[SUP] 6 [/SUP], Colin Parrish[SUP] 4 [/SUP], Stacey Schultz-Cherry[SUP] 2 [/SUP]
Affiliations
Abstract
Influenza virus isolation from clinical samples is critical for the identification and characterization of circulating and emerging viruses. Yet efficient isolation can be difficult. In these studies, we isolated primary swine nasal and tracheal respiratory epithelial cells and developed immortalized cell lines (siNEC and siTEC) that retained the abilities to form tight junctions and cilia and to differentiate at the air-liquid interface like primary cells. Critically, both human and swine influenza viruses replicated in the immortalized cells, which generally yielded higher-titer viral isolates from human and swine nasal swabs, supported the replication of isolates that failed to grow in MDCK cells, and resulted in fewer dominating mutations during viral passaging compared to MDCK cells.IMPORTANCERobust, in vitro culture systems for influenza virus are critically needed. Madin-Darby canine kidney (MDCK) cells, the most widely used cell line for influenza isolation and propagation, do not adequately model the respiratory tract. Therefore, many clinical isolates, both animal and human, are unable to be isolated and characterized, limiting our understanding of currently circulating influenza viruses. We have developed immortalized swine respiratory epithelial cells that retain the ability to differentiate and can support influenza replication and isolation. These cell lines can be used as additional tools to enhance influenza research and vaccine development.
. 2020 Sep 23;JVI.01091-20.
doi: 10.1128/JVI.01091-20. Online ahead of print.
Primary swine respiratory epithelial cell lines for the efficient isolation and propagation of influenza A viruses
Victoria Meliopoulos[SUP] 1 [/SUP], Sean Cherry[SUP] 2 [/SUP], Nicholas Wohlgemuth[SUP] 2 [/SUP], Rebekah Honce[SUP] 2 3 [/SUP], Karen Barnard[SUP] 4 [/SUP], Phillip Gauger[SUP] 5 [/SUP], Todd Davis[SUP] 5 [/SUP], Peter Shult[SUP] 6 [/SUP], Colin Parrish[SUP] 4 [/SUP], Stacey Schultz-Cherry[SUP] 2 [/SUP]
Affiliations
- PMID: 32967961
- DOI: 10.1128/JVI.01091-20
Abstract
Influenza virus isolation from clinical samples is critical for the identification and characterization of circulating and emerging viruses. Yet efficient isolation can be difficult. In these studies, we isolated primary swine nasal and tracheal respiratory epithelial cells and developed immortalized cell lines (siNEC and siTEC) that retained the abilities to form tight junctions and cilia and to differentiate at the air-liquid interface like primary cells. Critically, both human and swine influenza viruses replicated in the immortalized cells, which generally yielded higher-titer viral isolates from human and swine nasal swabs, supported the replication of isolates that failed to grow in MDCK cells, and resulted in fewer dominating mutations during viral passaging compared to MDCK cells.IMPORTANCERobust, in vitro culture systems for influenza virus are critically needed. Madin-Darby canine kidney (MDCK) cells, the most widely used cell line for influenza isolation and propagation, do not adequately model the respiratory tract. Therefore, many clinical isolates, both animal and human, are unable to be isolated and characterized, limiting our understanding of currently circulating influenza viruses. We have developed immortalized swine respiratory epithelial cells that retain the ability to differentiate and can support influenza replication and isolation. These cell lines can be used as additional tools to enhance influenza research and vaccine development.