Giuseppe
Emeritus
[Source: Journal of Virology, full page: (LINK). Abstract, edited.]
Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice
Yipeng Sun a, Qi Xu a,b, Ye Shen a, Linqing Liu a, Kai Wei a, Honglei Sun a, Juan Pu a, Kin-Chow Chang c and Jinhua Liu a#
Author Affiliations: <SUP>a</SUP> Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China <SUP>b</SUP> Veterinary Diagnostic Laboratory, China Animal Disease Control Center, Beijing, China <SUP>c</SUP> School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, United Kingdom
Published ahead of print 12 February 2014, doi: 10.1128/JVI.03158-13 <CITE>JVI.03158-13 </CITE>
<CITE></CITE>
<CITE></CITE>
<CITE></CITE>ABSTRACT
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicity in mice. We found that four amino acid mutations (PA-P224S, PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. Particularly, PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced its 50% mouse lethal dose (LD<SUB>50</SUB>) by almost 1,000-fold.
FOOTNOTES
#Corresponding author. Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China. Tel: +86-10-62733837; Fax: +86-10-62733837; E-mail: ljh@cau.edu.cn
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
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Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice
Yipeng Sun a, Qi Xu a,b, Ye Shen a, Linqing Liu a, Kai Wei a, Honglei Sun a, Juan Pu a, Kin-Chow Chang c and Jinhua Liu a#
Author Affiliations: <SUP>a</SUP> Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China <SUP>b</SUP> Veterinary Diagnostic Laboratory, China Animal Disease Control Center, Beijing, China <SUP>c</SUP> School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, United Kingdom
Published ahead of print 12 February 2014, doi: 10.1128/JVI.03158-13 <CITE>JVI.03158-13 </CITE>
<CITE></CITE>
<CITE></CITE>
<CITE></CITE>ABSTRACT
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicity in mice. We found that four amino acid mutations (PA-P224S, PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. Particularly, PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced its 50% mouse lethal dose (LD<SUB>50</SUB>) by almost 1,000-fold.
FOOTNOTES
#Corresponding author. Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China. Tel: +86-10-62733837; Fax: +86-10-62733837; E-mail: ljh@cau.edu.cn
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
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